Millipore

ChromoCult® Listeria agar (base) is used for the isolation and differentiation of Listeria monocytogenes and other Listeria sp. from food and animal feed, environmental samples in the area of food production and food handling, and other materials.

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Agency/Method	APHA, ISO 11290
Quality Level	200,
reg. compliance	FDA (BAM)
form	medium granules (dehydrated (DCM))
Торговая марка	ChromoCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.0-7.4 (25 °C, 70 g/L in H₂O)
solubility	70 g/L
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Listeria spp.

WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

ChromoCult® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L--phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

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ChromoCult® Listeria Agar Enrichment-Supplement

ChromoCult® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult® Listeria Selective Agar for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

Linkage

Replaces: 1004390017

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +2°C to +8°C.
Agency/Method	APHA, ISO 11133, ISO 11290-1, ISO 11290-2
Quality Level	100,
form	lyophilized powder
Торговая марка	ChromoCult^®
application(s)	microbiological culture: suitable, microbiology: suitable, pathogen testing: suitable
pH	6.6 (20 °C in H₂O)
Featured Industry	Food and Beverages
storage temp.	2-8°C
suitability	selective for Listeria spp. (Agar)

ChromoCult® Listeria Agar Selective-Supplement contains a mixture of four antibiotics in lyophilized form. It largely inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp. Amphotericin B reduces the growth of yeasts and molds. Polymyxin B inhibits the growth of Gram-negative bacteria. While nalidixic acid sodium salt mainly inhibits the growth of Gram-negative bacteria, it also inhibits some Gram-positive bacteria. Ceftazidime has a broad-spectrum activity against both Gram-negative and Gram-positive bacteria.

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ChromoCult® Listeria Agar Selective-Supplement

FRASER Listeria Selective Supplement is an additive for the preparation of ChromoCult® Listeria agar (base) for the selective growth of Listeria spp.

Linkage

Replaces: 1.00432.0017
Analysis Note
Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +2°C to +8°C.
Agency/Method	ISO 11290
Quality Level	100,
form	lyophilized powder
composition	Amphotericin B, 5 mg per vial ","Ceftazidime, 10 mg per vial ","Nalidixic acid sodium salt, 10 mg per vial ","Polymyxin B sulphate, 38350 (U per vial)
packaging	pkg of 10 vials
application(s)	microbiological culture: suitable","microbiology: suitable
pH	6.2 (20 °C in H₂O, Aqueous solution)
Featured Industry	Food and Beverages
compatibility	ChromoCult^® Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)
storage temp.	2-8°C
suitability	Listeria spp.

Chromocult® Tryptone Bile X-glucuronide agar is a selective medium for the enumeration of Escherichia coli. The presence of the enzyme -D-glucuronidase differentiates most E. coli from other coliforms. E. coli absorbs the chromogenic substrate 5-bromo-4-chloro-3- indolyl--D-glucuronide (X--D-glucuronide) while, the enzyme -glucuronidase splits the bond between the chromophore 5-bromo-4-chloro-3-indolyl and the -D-glucuronide. The formed E. coli colonies are colored blue to blue green. The growth of accompanying Gram-positive flora is largely inhibited due to the presence of bile salts and the high incubation temperature of 44°C. Some E. coli (3-4 %) are -D-glucuronidase negative and appear as colorless colonies (e.g. most E. coli O157 strains) or do not grow at the elevated temperature of 44°C (e.g. E. coli 0157:H7).

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Chromocult® Tryptone Bile X-glucuronide agar

Chromocult® Tryptone Bile X-glucuronide agar is used for the enumeration, detection, and identification of -glucuronidase positive Escherichia coli from food and feeding stuff, as well as from environmental samples in the area of food production and food handling.

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
Agency/Method	ISO 16649
form	medium granules (dehydrated (DCM))
Торговая марка	ChromoCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.0-7.4 (25 °C, 36.6 g/L in H₂O, after autoclaving)
solubility	31.6 g/L
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Escherichia coli

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Clear Fluid Thioglycollate Medium STBMCTM12

eCl@ss:	32014001
NACRES:	NA.74

Clear Thioglycollate medium has the same growth promotion properties as the standard Fluid Thioglycollate Medium (FTM). The alternative formulation brings extra visual clarity versus the FTM which has a slight turbidity or haze. Composition and growth performance of this medium comply with the European Pharmacopeia (EP) 2.6.1, United States Pharmacopeia (USP) 71 and Japanese Pharmacopeia (JP) 4.06. Our manufacturing approach ensures the highest level of quality for our media, improving accuracy and significantly reducing the risk of incorrect interpretation and false results. The appearance of the fluid is light yellow and viscous liquid with a pink ring in suspension ≤1 cm. The pH of the product is 7.1 ± 0.2 and can be stored at 2-25°C for 12 months. The incubation temperature varies between 30-35°C, with an incubation time of 7-14 days. One box of product contains 12 bottles of 100mL of Medium. Each bottle has a screw cap with a septum that minimizes the risks of cross-contamination and optimizes the disinfection procedures.

Clear Fluid Thioglycollate Medium is intended for the detection of anaerobic bacteria however, it also enables aerobic bacterial detection. This medium is used for sterility testing by membrane filtration or direct inoculation. The QC organisms include Clostridium sporogenes (ATCC 19404), Staphylococcus aureus (ATCC 6538) and Pseudomonas aeruginosa (ATCC 9027).
Sterility testing (membrane filtration and direct inoculation)
Bioburden testing

Incubation Time: 7–14 days

Screw cap with septum

Specifications

STERITEST is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Room Temperature (2 to 25 °C)
Quality Level	400,
Agency/Method	in accordance with EP 2.6.1, in accordance with JP 4.06, in accordance with USP 71
sterility	sterile; autoclaved
form	liquid
shelf life	12 mo.
feature	ready-to-use
packaging	pkg of 100 mL (12 Bottles with screw cap and septum)
application(s)	sterility testing: suitable
volume size	100 mL
input	sample type pharmaceutical(s)
pH	7.1±0.2 (25.0 °C)
test parameters	sample volume: 100 mL
Featured Industry	Cosmetics Food and Beverages Pharmaceutical
compatibility	for use with Steritest^®
shipped in	ambient
storage temp.	2-25°C
suitability	aerobic bacteria, nonselective for

190,18 евро / 19 432 руб.

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Clear Fluid Thioglycollate Medium STBMCTM12DP

eCl@ss:	32014001
NACRES:	NA.74

Clear Thioglycollate medium has the same growth promotion properties as the standard Fluid Thioglycollate Medium (FTM). The alternative formulation brings extra visual clarity versus the FTM which has a slight turbidity or haze. Composition and growth performance of this medium comply with the European Pharmacopeia (EP) 2.6.1, United States Pharmacopeia (USP) 71 and Japanese Pharmacopeia (JP) 4.06. Our manufacturing approach ensures the highest level of quality for our media, improving accuracy and significantly reducing the risk of incorrect interpretation and false results. The sterilized double Tyvek® packaging allows to streamline the cleaning procedures, to minimize the risk of cross-contamination in laminar flow hoods and to secure an efficient decontamination of isolator chambers. The appearance of the fluid is light yellow and viscous liquid with a pink ring in suspension 1 cm. The pH of the product is 7.1 ± 0.2 and can be stored at 2-25°C for 12 months. The incubation temperature varies between 30-35°C, with an incubation time of 7-14 days. One box of product contains 12 bottles of 100ml of Medium. Each bottle has a screw cap with a septum that minimizes the risks of cross-contamination and optimizes the disinfection procedures.

Clear Fluid Thioglycollate

Screw cap with septum

Culture Medium

Turbidity indicates growth

STERITEST is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

storage conditions	Room Temperature (2 to 25 °C)
Quality Level	400,
Agency/Method	in accordance with EP 2.6.1, in accordance with JP 4.06, in accordance with USP 71
sterility	sterile; ethylene oxide treated
form	liquid
feature	ready-to-use
packaging	pkg of 100 mL (12 Bottles with screw cap and septum, Double packed)
application(s)	sterility testing: suitable
volume size	100 mL
input	sample type pharmaceutical(s)
pH	7.1±0.2 (25.0 °C)
test parameters	sample volume: 100 mL
Featured Industry	Cosmetics Food and Beverages Pharmaceutical
compatibility	for use with Steritest^®
shipped in	ambient
storage temp.	2-30°C
suitability	anaerobic bacteria, nonselective for

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

CLED Agar is commonly used in bacteriological analysis.
CLED (cysteine-, lactose- and electrolyte-deficient) Agar is recommended for isolation, enumeration and identification of urinary pathogens.

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CLED Agar

CLED Agar has been used as a non-selective media to perform enantiomeric profiling of quinolones and quinolones resistance gene qnrS in wastewater samples

Suspend 36.15g in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , beef extract, 3 g/L , bromo thymol blue, 0.02 g/L , casein enzymic hydrolysate, 4 g/L , L-cystine, 0.128 g/L , lactose, 10 g/L , peptic digest of animal tissue, 4 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbe id \| utilization test: suitable, microbiological culture: suitable, microbiology: suitable
final pH	7.3±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
suitability	nonselective and differential for Enterococcus spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Lactobacillus spp., nonselective and differential for Proteus spp., nonselective and differential for Pseudomonas spp., nonselective and differential for Salmonella spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for coliforms

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

For the MPN enumeration of all clostridia in food and other material, for the cultivation and identification of Vibrio species from foods.

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Clostridial Differential Broth

Dissolve 27.5 g in 1 litre distilled water and fill into tubes. Sterilize by autoclaving at 121°C for 15 minutes.

Suitable for the detection of sulfite-reducing, spore-forming anaerobes (Clostridia).

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	ammonium ferric citrate, 0.5 g/L , casein peptone, 5.0 g/L , L-cysteine hydrochloride, 0.5 g/L , D(+)-glucose, 1.0 g/L , meat extract, 8.0 g/L , meat peptone, 5.0 g/L , resazurine sodium salt, 0.002 g/L , sodium acetate, 5.0 g/L , sodium disulfite, 0.5 g/L , starch, 1.0 g/L , yeast extract, 1.0 g/L
Торговая марка	NutriSelect^® Basic
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable, water monitoring: suitable
final pH	7.1±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
suitability	nonselective and differential for Clostridium spp.

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

For the cultivation and enumeration of clostridia and other anaerobes as well as facultative microorganisms in food, clinical and other material acc. to Hirsch & Grinsted.

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Clostridial Nutrient Medium

Dissolve 33 g in 1 litre water and sterilize by autoclaving at 121°C for 15 minutes.

Thermal resistance of non-proteolytic type B and type E Clostridium botulinum spores

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 0.5 g/L , L-cysteine hydrochloride, 0.5 g/L , D(+)-glucose, 5.0 g/L , meat extract, 10.0 g/L , peptone, 5.0 g/L , sodium acetate, 3.0 g/L , sodium chloride, 5.0 g/L , starch, 1.0 g/L , yeast extract, 3.0 g/L
Торговая марка	NutriSelect^® Basic
application(s)	microbiological culture: suitable, microbiology: suitable, sample preparation: suitable
final pH	6.8±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages
suitability	anaerobic bacteria, nonselective for Bacillus spp., nonselective for Clostridium spp., nonselective for Escherichia coli, nonselective for Staphylococcus spp., nonselective for coliforms

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

An antibiotic supplement for the selective isolation of Clostridium difficile.

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Clostridium difficile Supplement

Composition (per vial; sufficient for 500 mL medium)
D-Cycloserine 250 mg; Cefoxitin 8 mg

sterility	sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
application(s)	microbiology: suitable
Featured Industry	Clinical Testing Environmental Food and Beverages
storage temp.	2-8°C
suitability	Clostridium spp.

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P280
Personal Protective Equipment	dust mask type N95 (US),, Eyeshields,, Faceshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Clostridium perfringens selective supplement contains the antibiotic D-Cycloserine that inhibits the accompanying bacterial flora and causes the developing colonies to remain smaller. It also reduces the diffusion and the disturbing blackening around the Clostridium perfringens colonies. 4-Methylumbelliferylphosphate (MUP) is a fluorogenic substrate for the alkaline and acid phosphatase. The acid phosphatase is a high specific indicator for Clostridium perfringens, and it splits the fluorogenic substrate MUP. The fluorescence of resulting product, 4-methylumbelliferone, can be identified in long wave UV-light, which suggests the presence of Clostridium perfringens.

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Clostridium perfringens selective supplement

Clostridium perfringens selective supplement is used for the enrichment of TSC-Agar (Base) during the enumeration of Clostridium perfringens in food products and other materials.

Linkage

Replaces: 1008880017

storage conditions	Store at +2°C to +8°C.
Quality Level	200,
form	lyophilized powder
application(s)	microbiological culture: suitable, microbiology: suitable
solubility	83 g/L
Featured Industry	Food and Beverages
storage temp.	2-8°C
suitability	Clostridium spp.

Coliform ChromoSelect Agar is a chromogenic mixture which contains two chromogenic substrates, Salmon-GAL and X-glucuronide. Coliforms produce an enzyme called -D-galactosidase, which cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme -D-glucuronidase produced by Escherichia coli cleaves X-glucuronide. Escherichia coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction, thereby increasing detection reliability in combination with the two chromogens. To confirm Escherichia coli, add a drop of Kovacs reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

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Coliform ChromoSelect Agar

Coliform ChromoSelect Agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms in water and food samples.

Suspend 27 g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. When a high number of gram-positive accompanying bacteria are expected, add 5 mg/l Novobiocin (Cat. No. 74675) before autoclaving of the medium.

Reconstitution

Suspend 27 g in 1 liter distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121 °C for 15 minutes. When a high number of Gram-positive accompanying bacteria are expected, add 5 mg/L Novobiocin (Catalog No. 74675) before autoclaving of the medium.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 12 g/L , chromogenic mixture, 0.2 g/L , dipotassium hydrogen phosphate, 3 g/L , peptone, special, 3 g/L , potassium dihydrogen phosphate, 1.7 g/L , sodium chloride, 5 g/L , sodium lauryl sulfate, 0.1 g/L , sodium pyruvate, 1 g/L , tryptophan, 1 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	6.8±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
suitability	selective and differential for Citrobacter spp., selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Coliform ChromoSelect Agar is recommended for the simultaneous detection of Escherichia coli and thermotolerent coliforms in water, milk, dairy products and other food samples.

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Coliform ChromoSelect Agar, Modified

Suspend 24 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

form	powder
Quality Level	100,
shelf life	limited shelf life, expiry date on the label
composition	agar, 10.0 g/L , bile salts, 0.80 g/L , chromogenix mixture, 0.20 g/L , dipotassium phosphate, 0.60 g/L , magnesium sulfate, 0.20 g/L , peptone, special, 8.0 g/L , potassium dihydrogen phosphate, 0.20 g/L , sodium chloride, 1.0 g/L , yeast extract, 3.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	7.20±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
storage temp.	2-8°C
suitability	Escherichia coli, coliforms

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Appearance
- clearness:
clear
- colour: yellowish
pH-value (25°C): 6.6 - 7.0
Inoculum on reference medium
- Escherichia coli ATCC 11775 (WDCM 00090):
10 - 100
- Citrobacter freundii ATCC 8090: 10 - 100
- Klebsiella pneumoniae ATCC 31488: 10 - 100
- Salmonella typhimurium ATCC 14028 (WDCM 00031): 10 - 100
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): 1000
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): +
colour change to blue-green
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Fluorescence
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Indole formation
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
Incubation: 24 hrs.; 35 °C; aerobic.

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Coliforms 50

READYCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
sterility	sterile; -irradiated
form	medium granules (dehydrated (DCM))
Торговая марка	ReadyCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	6.6-7.0 (25 °C, 17 g/L in H₂O)
solubility	17 g/L
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Escherichia coli, coliforms

This high-grade complete medium acc. to Ellner is for the cultivation of fastidious microorganisms. It is also used as a base for the preparation of a variety of special media.

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Columbia Agar

Dissolve 42 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Base agar in a medium for isolating Listeria spp. from heavily contaminated material; In a simple plate assay for detection of group A streptococcus proteinase.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Agency/Method	according to ISO 10272-1:2017, according to ISO 10272-2:2017
Quality Level	200,
sterility	non-sterile
product line	BioChemika
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , sodium chloride, 5.0 g/L , special nutrient substrate, 23.0 g/L , starch, 1.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbe id \| specific enzyme detection: suitable, microbiological culture: suitable, microbiology: suitable (microbial identification)
final pH	7.3±0.2 (25 °C)
Featured Industry	Clinical Testing Cosmetics Environmental Food and Beverages Veterinary
suitability	Gardnerella spp., Haemophilus spp., Pneumococcus spp., nonselective for Bacteroides spp., nonselective for Campylobacter spp., nonselective for Clostridium spp., nonselective for Corynebacterium spp., nonselective for Enterococcus spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

For the primary cultivation of aerobic, microaerophilic and anaerobic bacteria from clinical specimens.

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Cooked Meat Broth

Suspend 57 g in 1 litre distilled water. Mix well and allow to stand for 15 minutes until all the particles are thoroughly wetted. Mix well and while maintaining an even suspension, distribute into tubes. Sterilize by autoclaving at 121°C for 15 minutes.

For the cultivation and isolation of Clostridium difficile.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	beef heart (extract from 454g), 30 g/L , D(+)-glucose, 2 g/L , meat peptone (peptic or mixed peptone), 20 g/L , sodium chloride, 5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.2±0.2 (25 °C)
Featured Industry	Agriculture Clinical Testing Environmental Food and Beverages Life Science and Biopharma
storage temp.	2-8°C
suitability	anaerobic bacteria, nonselective for Bacteroides spp., nonselective for Clostridium spp., nonselective for Enterococcus spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Corn Meal Agar is recommended for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures.

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Corn Meal Agar

Corn Meal Agar has been used as a component of phytophthora selective agar for the isolation of phytophthora from strawberry crowns.

Suspend 17g in 1 litre of distilled water. Bring to the boil to dissolve completely. If desired add 1% polysorbate 80. Sterilise by autoclaving at 121°C for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	Agar, 15 g/L , Corn meal infusion (from 50 g whole maize), 2 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable (microbial identification)
pH	6.0±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages Life Science and Biopharma
suitability	nonselective for Aspergillus spp., nonselective for Candida spp., nonselective for Penicillium spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., nonselective for molds (General Media), nonselective for yeasts (General Media)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Appearance (clearness): clear to slightly opalescent
Appearance (colour): yellowish
pH-value (25°C): 7.3 - 7.7
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): 70 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 70 %
Recovery on test medium (Enterococcus faecalis ATCC 11700): 70 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): 70 %
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): 70 %
Incubation: 48 hrs.; 35 °C; aerobic.

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Count agar sugar free FIL-IDF

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.4-7.6 (37 °C, 35 g/L in H₂O, after autoclaving)
solubility	35 g/L
bulk density	700 kg/m³
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	infectious bacteria (in dairy)

A chromogenic Agar for enumeration detection and differentiation of Clostridium sp., in particular Clostridium perfringens, in aqueous samples. The medium is more reliable than m-CP Agar and easier to handle.

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CP ChromoSelect Agar

Suspend 31.42 g in 400 ml distilled water and adjust the volume to 500 ml. Boil to dissolve the medium completely. Cool to 50-55°C and add 1 vial of Perfringens T.S.C. Supplement (P9352) or 1 vials of M-CP selective Supplement I (Cat. No. 51962). Dispense into petri dishes (we recommend small ones).

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , ammonium iron(III) citrate, 0.2 g/L , chromogenic mixture, 1.73 g/L , L-cysteine hydrochloride, 1.0 g/L , magnesiumsulfate heptahydrate, 0.1 g/L , soy peptone, 5.0 g/L , sucrose, 3.0 g/L , tris buffer, 1.8 g/L , tryptose (vegetable), 20.0 g/L , yeast extract, 15.0 g/L
Торговая марка	NutriSelect^® Basic
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification), water monitoring: suitable
final pH	7.4±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages
suitability	selective and differential for Clostridium spp.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Cellobiose-polymyxin B-colistin(CPC) agar is a medium used for the isolation and differentiation of Vibrio vulnificus in laboratory testing.

Подробнее

CPC Selective Supplement

Кат. номер

17137-5VL

17137-1VL

17137-1EA

Application
For selective isolation and enumeration of vibrio species from foods.
Components
Composition (per vial; sufficient for 500 mL medium)
Colistin methane sulfonate 63 mg; Polymyxin B sulfate 9 mg
Параметры

sterility sterile

Quality Level 100,

form powder

application(s) microbiology: suitable

Featured Industry Food and Beverages
Food and Beverages

storage temp. 2-8°C

suitability Vibrio spp.

Safety Information

Personal Protective Equipment Eyeshields,, Gloves,, type N95 (US),

RIDADR NONH for all modes of transport

WGK Germany WGK 3

Flash Point F Not applicable

Flash Point C Not applicable

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CPC-Agar (Base)

for the cultivation and identification of Vibrio species from foods

Suspend 32.54 g in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45°C and aseptically add reconstituted contents of 1 vial of CPC Supplement (Cat. No. 17137). Mix well.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
composition	agar, 15 g/L , beef extract, 5 g/L , bromothymol blue, 0.04 g/L , cellobiose, 15 g/L , cresol red, 0.04 g/L , peptic digest of animal tissue, 10 g/L , sodium chloride, 20 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification)
final pH	7.6±0.2 (25 °C)
Featured Industry	Food and Beverages
suitability	selective and differential for Vibrio spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

An enrichment broth for the detection of Cronobacter (formerly E. sakazakii) from food and environmental samples.

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Cronobacter screening broth

Dissolve 28 g in 1000 ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (Cat. No. 75423) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
Agency/Method	according to ISO 22964:2017
form	powder
shelf life	limited shelf life, expiry date on the label
composition	bromocresol purple, 4 mg/L , meat extract, 3.0 g/L , peptone, 10.0 g/L , sodium chloride, 5.0 g/L , sucrose, 10.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification)
final pH	7.4±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
suitability	Cronobacter spp.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Cronobacter spp. ChromoSelect Agar is recommended for isolation and identification of Cronobacter spp. (fomerly Enterobacter sakazakii) from food, water, sewage, urine and feces samples.

Подробнее

Cronobacter spp. ChromoSelect Agar

Suspend 51.67 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 Ibs pressure (121 °C) for 15 minutes. Cool to 45-50°C and pour into sterile petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , casein enzymic hydrolysate, 15.0 g/L , chromogenic mixture, 10.17 g/L , papaic digest of soyabean meal, 5.0 g/L , sodium chloride, 5.0 g/L , sodium deoxycholate, 0.5 g/L , sodium thiosulfate, 1.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable (microbial identification)
final pH	7.3±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages Veterinary
storage temp.	2-8°C
suitability	selective and differential for Cronobacter spp., selective and differential for Enterobacter spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Cronobacter spp. ChromoSelect Agar is recommended for the isolation and identification of Cronobacter spp. from milk and milk products.

Подробнее

Cronobacter spp. ChromoSelect Agar, modified

Suspend 30.75 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 Ibs pressure (121°C) for 15 minutes. Cool to 45-50°C and pour into sterile petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , casein enzymic hydrolysate, 7.0 g/L , chromogenic substrate, 0.15 g/L , crystal violet, 0.002 g/L , sodium chloride, 5.0 g/L , sodium deoxycholate, 0.6 g/L , yeast extract, 3.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiology: suitable (microbial identification)
final pH	7.0±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
storage temp.	2-8°C
suitability	Cronobacter spp., selective and differential for Cronobacter spp., selective and differential for Enterobacter spp.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Подробнее

CSB (Cronobacter Selective Broth) 1205970500

acc. ISO 22964 GranuCult®

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Agency/Method	according to ISO 22964
Quality Level	200,
form	medium granules
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Cronobacter spp. (spp.)

WGK Germany

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CSB Selective Supplement (Vancomycin)

Empirical Formula (Hill Notation):	C₆₆H₇₅Cl₂N₉O₂₄ · xHCl
CAS Number:	1404-93-9
Molecular Weight:	1449.25 (free base basis)
EC Index Number:	215-772-6

storage conditions	Store at +2°C to +8°C.
Agency/Method	ISO
form	lyophilized powder
application(s)	microbiology: suitable
pH	2.5-4.5 (20 °C, 50 g/L in H₂O)
Featured Industry	Food and Beverages
shipped in	ambient
storage temp.	2-8°C
suitability	Cronobacter spp.

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

A selective supplement recommended by ISO Committee for the isolation of Escherichia coli 0157.

Подробнее

CT Supplement

(one vial sufficient for 500 mL medium) Potassium tellurite 1.25 mg, Cefixime 0.025 mg

sterility	sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
application(s)	microbiology: suitable
Featured Industry	Agriculture Environmental Food and Beverages
suitability	Escherichia coli, coliforms

pictograms	GHS06
signalword	Danger
hcodes	H301 - H315 - H317 - H319
pcodes	P280 - P301 + P310 + P330 - P302 + P352 - P305 + P351 + P338
Personal Protective Equipment	Eyeshields,, Faceshields,, Gloves,, type P2 (EN 143) respirator cartridges,
RIDADR	UN 3284 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

A semi-synthetic solid medium for the general cultivation of fungi, yeasts and soil bacteria.

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Czapek Dox Agar

Suspend 50 g in 1 litre of distilled water. Bring to the boil in order to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes. Mix well before pouring.

Assay of capstan using B. megaterium.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , di-potassium hydrogen phosphate, 1.0 g/L , iron(II) sulfate heptahydrate, 0.01 g/L , magnesium sulfate heptahydrate, 0.5 g/L , potassium chloride, 0.5 g/L , sodium nitrate, 3.0 g/L , sucrose, 30.0 g/L
Торговая марка	NutriSelect^® Basic
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification)
final pH	7.3±0.2 (25 °C)
Featured Industry	Agriculture Environmental Food and Beverages Life Science and Biopharma
suitability	Arthrobacter spp., nonselective for Aspergillus spp., nonselective for Bacillus spp., nonselective for Candida spp., nonselective for Penicillium spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., nonselective for molds (General Media), nonselective for yeasts (General Media)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1

Czapek-Dox Agar is a semi-synthetic broth used for the cultivation of fungi, containing sodium nitrate as the sole source of nitrogen.

Подробнее

Czapek-Dox broth

Czapek-Dox broth has been used for culturing vegetative fugal mycelium, Aspergillus fumigatus strain 10AF and Verticillium albo-atrum.

Packaging

250 g in poly bottle
1 kg in poly bottle

Contains: Sucrose, sodium nitrate, dipotassium phosphate, magnesium sulfate, potassium chloride, ferrous sulfate.

Suspend 35.0 g/L in water, boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and dispense as desired

Microbiologically tested.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
Торговая марка	NutriSelect^® Basic
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
Featured Industry	Food and Beverages
suitability	nonselective for Aspergillus spp., nonselective for Candida spp., nonselective for Saccharomyces spp., nonselective for molds (General Media), nonselective for yeasts (General Media)

pictograms	GHS03
signalword	Warning
hcodes	H272
pcodes	P210 - P220 - P280 - P370 + P378 - P501
RIDADR	UN 1479 5.1 / PGIII
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

for the isolation and characterization of nutrional mutants of Escherichia coli

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Davis Minimal Agar

Suspend 26.6 g in 1000 ml of distilled water. Bring to the boil and dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile petri dishes or dispense as desired.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , ammonium sulfate, 1 g/L , dextrose, 1 g/L , dipotassium phosphate, 7 g/L , magnesium sulfate, 0.1 g/L , monopotassium phosphate, 2 g/L , sodium citrate, 0.5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.0±0.2 (25 °C)
Featured Industry	Food and Beverages Life Science and Biopharma
suitability	nonselective for Escherichia coli, nonselective for coliforms

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Davis Minimal Broth is recommended for the isolation and characterization of nutrional mutants of Escherichia coli.

Подробнее

Davis Minimal Broth

Suspend 11.6 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Mix well.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	ammonium sulfate, 1 g/L , dextrose, 1 g/L , dipotassium phosphate, 7 g/L , magnesium sulfate, 0.1 g/L , monopotassium phosphate, 2 g/L , sodium citrate, 0.5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.0±0.2 (25 °C)
Featured Industry	Food and Beverages Life Science and Biopharma
suitability	nonselective for Escherichia coli, nonselective for coliforms

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Appearance
- clearness:
clear
- colour: yellowish-Brown to reddish-Brown
pH-value (25°C): 7.4 - 7.8
Growth
- Clostridium perfringens ATCC 10543:
good to very good
- Clostridium perfringens ATCC 13124 (WDCM 00007): good to very good
- Clostridium sporogenes ATCC 19404 (WDCM 00008): good to very good
- Clostridium bifermentans ATCC 19299: good to very good
- Bacillus licheniformis ATCC 14580 (WDCM 00068): poor to fair
Blacking
- Clostridium perfringens ATCC 10543:
+
- Clostridium perfringens ATCC 13124 (WDCM 00007): +
- Clostridium sporogenes ATCC 19404 (WDCM 00008): +
- Clostridium bifermentans ATCC 19299: +
- Bacillus licheniformis ATCC 14580 (WDCM 00068): -
Recovery on test medium
- Clostridium perfringens ATCC 10543:
70 %
- Clostridium perfringens ATCC 13124 (WDCM 00007): 70 %
- Clostridium sporogenes ATCC 19404 (WDCM 00008): 70 %
- Clostridium bifermentans ATCC 19299: 70 %
Incubation: 48 hrs.; 30 °C with Anaerocult A.

Подробнее

DCA agar acc. to Weenk et al.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.4-7.8 (25 °C in H₂O)
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Clostridium spp.

A medium for the culture of pathogenic Vibrio species as well as Salmonella and Shigella.

Подробнее

DCLS Agar

Suspend 50 g in 1 litre of distilled water. Bring to the boil to dissolve the medium completely. Cool to 50°C and pour plates. Do not autoclave!

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

form	powder
Quality Level	200,
shelf life	limited shelf life, expiry date on the label
composition	agar, 12.0 g/L , lactose, 5.0 g/L , neutral red, 0.03 g/L , sodium citrate, 10.5 g/L , sodium desoxycholate, 2.5 g/L , sodium thiosulfate, 5.0 g/L , special peptone, 10.0 g/L , sucrose, 5.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.2±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
suitability	selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Vibrio spp., selective and differential for Yersinia spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Подробнее

De Man, Rogosa and Sharpe Broth MHA00MRS2

eCl@ss:	32160801
NACRES:	NA.74

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus. The MRS media formulation was developed by de Man, Rogosa and Sharpe {23} to replace the tomato juice medium and the meat extract tomato juice medium. It is a medium supporting good growth of lactobacilli in general, even those strains which have shown poor growth in existing media, like strains of L. brevis and L. fermenti. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species as well as other secondary bacteria may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide and polymyxin. Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies.{24}

MRS Broth is used for final product testing or in-process testing of beverages for the contamination by lactobacilli, L. brevis and L. fermenti in Beer, Bottled Water, Cider, Soft Drink and Sport Drink. This media finds application in Clinical, Cosmetics and Food and Beverage industry.

Packaging

50 pk in 2 mL plastic ampoules

Culture Medium

MRS

QC organisms
L. casei (ATCC 393)
Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	2 to 10 °C
Quality Level	100,
form	liquid
shelf life	12 mo.
feature	ready-to-use
packaging	ampule of 2 mL, pack of 50 tests
application(s)	microbiology: suitable, water monitoring: suitable
color	clear red-brown
input	sample type beverage(s), sample type food(s) sample type bottled water sample type wine sample type cider sample type cosmetics sample type beer sample type soft drink sample type sport drink
pH	6.5±0.2 (25.0 °C)
Featured Industry	Clinical Cosmetics Environmental Food and Beverages Industrial QC
compatibility	for use with MF-method, for use with Microfil^®, for use with MicropreSure^®, for use with Milliflex^®
shipped in	wet ice
storage temp.	2-8°C
suitability	Lactobacillus brevis, Lactobacillus fermenti, lactic acid bacteria

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Used to differentiate bacteria on the basis of their ability to decarboxylate amino acids.

Подробнее

Decarboxylase broth base, Moeller

Ingredients (g/L)
Peptic digest of animal tissue, 5.00
Beef extract, 5.00
Dextrose, 0.50
Bromocresol purple, 0.01
Cresol red, 0.005
Pyridoxal, 0.005

Suspend 10.52 g of Decarboxylase Broth Base in 1000 ml of distilled water. Add 10 g of either L-Arginine (Cat. No. 11010), or L-Lysine (Cat. No. 62840), or L-Ornithine (Cat. No. 75470). Heat to dissolve the medium completely. When L-ornithine is added, readjust the pH. Dispense in 5 ml quantities in screwcapped tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
product line	BioChemika
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbe id \| specific enzyme detection: suitable, microbiological culture: suitable, microbiology: suitable (microbial identification)
final pH	6.0±0.2 (25 °C)
Featured Industry	Clinical Testing Environmental Food and Beverages
suitability	nonselective and differential for Citrobacter spp., nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for Salmonella spp., nonselective and differential for Serratia spp., nonselective and differential for Shigella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

A selective medium recommended for the isolation of enteric pathogens particularly Salmonella and Shigella species.

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Deoxycholate citrate agar

Components (g/L)
Heart infusion solids 10.00
Proteose peptone 10.00
Lactose 10.00
Sodium citrate 20.00
Ferric ammonim citrate 2.00
Sodium deoxycholate 5.00
Neutral red 0.02
Agar 13.50

Suspend 70.52 g of Deoxycholate Citrate Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Do not autoclave. Avoid excessive heating as it is detrimental to the medium.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable (microbial identification)
pH	7.5±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
suitability	selective and differential for Salmonella spp., selective and differential for Shigella spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Deoxycholate lactose agar

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	6.9-7.3 (25 °C, 57 g/L in H₂O)
solubility	57 g/L
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	coliforms

A diagnostic medium for the detection of coagulase-positive DNase producing Staphylococci.

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Deoxyribonuclease Test Agar

In an identification scheme for coagulase negative staphylococci from bovine mastitis

sterility	non-sterile
Quality Level	100,
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , casein enzymic hydrolysate, 15 g/L , deoxyribonucleic acid, 2 g/L , papaic digest of soybean meal, 5 g/L , sodium chloride, 5 g/L
application(s)	microbiology: suitable
final pH	7.3±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages
suitability	nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	3

Appearance (clearness): clear to slightly turbid
Appearance (colour): yellowish-brown
Solidification behaviour (2hrs., 45°C): liquid
pH-value (25°C): 7.1 - 7.5
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): 70 %
Recovery on test medium (Proteus hauseri ATCC 13315): 70 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): 70 %
Recovery on test medium (Enterococcus faecalis ATCC 11700): 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): 70 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): 70 %
Recovery on test medium (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): 70 %
Incubation: 24 hrs.; 35 °C; aerobic.

Подробнее

DEV gelatin agar

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.1-7.5 (22 °C, 50 g/L in H₂O, after autoclaving)
solubility	50 g/L
bulk density	750 kg/m³
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	gelatin-liquefying bacteria

Appearance (clearness): clear
Appearance (colour): violet
pH-value (25°C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Proteus hauseri ATCC 13315): good to very good
Growth (Enterococcus faecalis ATCC 11700): good to very good
Colour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013)): +
Colour change to yellow (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Colour change to yellow (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Colour change to yellow (Proteus hauseri ATCC 13315): -
Colour change to yellow (Enterococcus faecalis ATCC 11700): +/-
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Gas (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Gas (Proteus hauseri ATCC 13315): -
Gas (Enterococcus faecalis ATCC 11700): -
Incubation: 40-48 hrs.; 35 °C; aerobic.

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DEV Lactose broth

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.1-7.3 (35 °C, 28 g/L in H₂O, after autoclaving)
solubility	28 g/L
bulk density	640 kg/m³
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Escherichia coli, coliforms

Appearance (clearness): clear
Appearance (colour): violet
pH-value (25°C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): medium to very good
Growth (Enterococcus faecalis ATCC 11700): medium to very good
Colour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013)): +
Colour change to yellow (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Colour change to yellow (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Colour change to yellow (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Colour change to yellow (Enterococcus faecalis ATCC 11700): +/-
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Gas (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Gas (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Gas (Enterococcus faecalis ATCC 11700): -
Incubation: 24 hrs.; 35 °C; aerobic.

Подробнее

DEV Lactose peptone broth

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	7.0 (37 °C, 25 g/L in H₂O, after autoclaving)
solubility	25 g/L
bulk density	700 kg/m³
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	coliforms

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25°C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Indole formation (Escherichia coli ATCC 11775 (WDCM 00090)): +
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Indole formation (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): -
Growth (Proteus mirabilis ATCC 14153): good to very good
Indole formation (Proteus mirabilis ATCC 14153): -
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Indole formation (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Incubation:4 -6 hrs.; 35°C; aerobic.

Подробнее

DEV TRYPTOPHAN BROTH

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
form	powder (dehydrated (DCM))
Quality Level	200,
Торговая марка	GranuCult^®
application(s)	microbe id \| specific enzyme detection: suitable, microbiological culture: suitable, microbiology: suitable
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	Escherichia coli, coliforms

Used to propagate Neisseria gonorrhoeae.

Подробнее

Dextrose starch agar

Ingredients(g/L)
Proteose peptone15.00
Dextrose2.00
Starch, soluble10.00
Sodium chloride5.00
Disodium phosphate3.00
Gelatin20.00
Agar10.00

Suspend 65 g of Dextrose Starch Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the tubed medium in a slanted position.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	100,
form	powder
shelf life	limited shelf life, expiry date on the label
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.3±0.2 (25 °C)
Featured Industry	Clinical Testing Food and Beverages
suitability	nonselective for Neisseria spp., nonselective for Streptococcus spp.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Dey-Engley Neutralizing Agar is used in disinfectant testing where neutralization of the antiseptics and disinfectants is important for determining its bactericidal activity.

Подробнее

Dey-Engley Neutralizing Agar

Suspend 54.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclavingat 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

sterility	non-sterile
Quality Level	200,
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , bromocresol purple, 0.02 g/L , casein enzymic hydrolysate, 5 g/L , dextrose, 10 g/L , lecithin, 7 g/L , polysorbate 80, 5 g/L , sodium bisulfite, 2.5 g/L , sodium thioglycollate, 1 g/L , sodium thiosulfate, 6 g/L , yeast extract, 2.5 g/L
Торговая марка	NutriSelect^® Plus
application(s)	microbiological culture: suitable, microbiology: suitable
final pH	7.6±.2 (25 °C)
Featured Industry	Environmental Food and Beverages Pharmaceutical
suitability	bacteria

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

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DG 18 (Dichloran glycerol chloramphenicol) agar (base)

Appearance (clearness): clear to slightly opalescent
Appearance (colour): yellowish
pH-value (25°C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): 50 %
Inoculum on reference medium (Wallemia sebi ATCC 42694 (WDCM00182)):
Colony count (Wallemia sebi ATCC 42694 (WDCM00182)):
Recovery on test medium (Wallemia sebi ATCC 42694 (WDCM00182)): 50 %
Inoculum on reference medium (Aspergillus caesiellus ATCC 42693 (WDCM00183)):
Colony count (Aspergillus caesiellus ATCC 42693 (WDCM00183)):
Recovery on test medium (Aspergillus caesiellus ATCC 42693 (WDCM00183)): 50 %
Inoculum on reference medium (Eurotium rubrum ATCC 42690 (WDCM00183)):
Colony count (Eurotium rubrum ATCC 42690 (WDCM00183)):
Recovery on test medium (Eurotium rubrum ATCC 42690 (WDCM00183)): 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no growth
Growth (Mucor racemosus ATCC 42647): good to very good
Growth (Penicillium cyclopium CMI 19759): good to very good
Growth (Rhizopus stolonifer ATCC 15441): good to very good
Incubation: 5 days at 25 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD dextrose agar

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	Store at +15°C to +25°C.
Quality Level	200,
Agency/Method	ISO
reg. compliance	FDA
form	medium granules (dehydrated (DCM))
Торговая марка	GranuCult^®
application(s)	microbiological culture: suitable, microbiology: suitable
pH	5.4-5.8 (25 °C in H₂O)
Featured Industry	Food and Beverages
storage temp.	15-25°C
suitability	molds, yeasts

Dichloran Glycerol Medium Base with Chloramphenicol is recommended for selective isolation of xerophilic moulds from food samples. It is a medium for enumeration of xerophilic fungi from low-moisture foods.

Подробнее

Dichloran Glycerol Agar

Dichloran Glycerol Agar has been used for culturing Aspergillus flavus AF57 on rice grains and fungal populations from conidial suspension treated rice grains.

Suspend 15.8 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Add 110 grams of glycerol (Analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Agency/Method	according to ISO 21527-2:2008
Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15.0 g/L , chloramphenicol, 0.1 g/L , dextrose, 10.0 g/L , dichloran, 0.002 g/L , magnesium sulfate, 0.5 g/L , monopotassium phosphate, 1.0 g/L , peptic digest of animal tissue, 5.0 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiological culture: suitable, microbiology: suitable
final pH	5.6±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
suitability	selective for molds (General Media), selective for yeasts (General Media)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

For selective isolation and enumeration of fungi-yeasts and molds of significance in food spoilage.

Подробнее

Dichloran Rose bengal Agar (Base)

Suspend 15.75 g in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add sterile reconstituted contents of 1 vial of Chloramphenicol Selective Supplement (Cat. No. 29231). Mix well and pour into sterile petri plates.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Agency/Method	according to ISO 21527-1:2008
Quality Level	200,
sterility	non-sterile
form	powder
shelf life	limited shelf life, expiry date on the label
composition	agar, 15 g/L , dextrose, 10 g/L , dichloran, 0.002 g/L , magnesium sulfate, 0.5 g/L , monopotassium phosphate, 1 g/L , peptic digest of animal tissue, 5 g/L , rose bengal, 0.025 g/L
Торговая марка	NutriSelect^® Plus
application(s)	bioburden testing: suitable, microbiology: suitable (microbial identification)
final pH	5.6±0.2 (25 °C)
Featured Industry	Environmental Food and Beverages
suitability	selective for Aspergillus spp., selective for Candida spp., selective for Penicillium spp., selective for Pichia spp., selective for Saccharomyces spp., selective for Zygosaccharomyces spp., selective for molds (General Media), selective for yeasts (General Media)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Appearance (clearness): clear
Appearance (colour): reddish-brown
pH-value (25°C): 6.9 - 7.3
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): poor to fair
Growth (Clostridium bifermentans ATCC 19299): good to very good
Growth (Clostridium perfringens ATCC 13124 (WDCM 00007)): good to very good
Growth (Clostridium perfringens ATCC 10543): good to very good
Growth (Clostridium sporogenes ATCC 11437): good to very good
Growth (Clostridium sporogenes ATCC 19404 (WDCM 00008)): good to very good
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Blacking (Clostridium bifermentans ATCC 19299): +
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007)): +
Blacking (Clostridium perfringens ATCC 10543): +
Blacking (Clostridium sporogenes ATCC 11437): +
Blacking (Clostridium sporogenes ATCC 19404 (WDCM 00008)): +
Incubation: 7 days; 30 °C; layered with paraffin.

Подробнее

Differential reinforced clostridial broth (DRCM)

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany