Anti-Versican Antibody, Beta Domain

eCl@ss:

32160702

Кат. номер

ABT1370

ABT1370-25UL

Versican core protein (UniProt: Q9ERB4; also known as Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M) is encoded by the Vcan (also known as Cspg2) gene (Gene ID: 114122) in rat. Versican is a large multi-domain chondroitin sulphate proteoglycan that is secreted by fibroblasts and other types of cells. Versican is present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer. It is synthesized with a 20 amino acid signal peptide, which is subsequently cleaved. Veriscan plays a role in intercellular signaling and in connecting cells with the extracellular matrix and is also involved in axon regeneration, central nervous system and skeletal muscle development, and cell adhesion. It has unique N- and C-terminal globular regions, each with multiple motifs. It contains one C-type lectin domain, two EGF-like domains, one Ig-like V-type domain, two link domains and one Sushi (CCP/SCR) domains. It also has a N-terminal hyaluronic acid (HA) binding domain that has homology with the G1 and G2 domains of aggrecan. Four isoforms of versican termed as V0, V1, V2, and V3, have been reported with molecular weights of 370, 263, 180, and 74 kDa, respectively. Each isoform contains different lengths of the GAG binding region. V0 contains two GAG binding regions called CSalpha and CSbeta domains. V1 isoform has only CSbeta and V2 isoform has only CSalpha domain. V3 isoform contains only G1 and G3 domains and lacks GAG attachment sites. Defects in Vcan gene result in Wagner syndrome type 1, a type of vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a pre-retinal avascular membrane.
GST-tagged recombinant fragment corresponding to the 375 amino acids from the internal region of rat Versican.
Anti-Versican, Beta Domain, Cat. No. ABT1370 is a rabbit polyclonal antibody that detects versican core protein and has been tested for use in Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.
Radioimmunoassay Analysis: A representative lot detected Versican, Beta Domain in Postnatal rat brain (Milev, P., et. al. (1998). Biochem Biopys Res Commun. 247(2):207-12).
Immunohistochemistry Analysis: A representative lot detected Versican, Beta Domain in Embryonic day 16 rat eye, P0, P9 and adult rat retina (Popp, S., et. al. (2004). Exp Eye Res. 79(3):351-6) and Embryonic day 16, 19 rat brain and postnatal day 7 cerebellum, embryonic day 19 spinal cord (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).
Western Blotting Analysis: A representative lot detected Versican, Beta Domain in puried proteoglycans from a PBS extract of 7-day rat brain with chondroitinase ABC treatment (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).
Quality
Evaluated by Immunohistochemistry in rat eye retina tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Versican, Beta Domain in rat eye retina tissue.
Target description
300 kDa calculated.
Concentration: Please refer to lot specific datasheet.

biological source	rabbit
Quality Level	100
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	rat
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable (paraffin)","radioimmunoassay: suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_001164029,
UniProt accession no.	Q9ERB4,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable