Anti-APN/CD13 Antibody, clone DF-5

eCl@ss:

32160702

Кат. номер

MABF2078-25UG

MABF2078-100UG

Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967).
Specificity
Clone DF-5 specifically detects aminopeptidase N in human cells.
Crude cell membrane fraction from adult human papillary and reticular dermal fibroblasts.
This mouse monoclonal Anti-APN/CD13, clone DF-5, Cat. No. MABF2078, detects Aminopeptidase N and has been tested for use in Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in human prostate tissue.
Flow Cytometry Analysis: A representative lot detected APN/CD13 in Flow Cytometry applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).
Immunofluorescence Analysis: A representative lot detected APN/CD13 in Immunofluorescence applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23; Sorrell, J.M., et. al. (2007). Cell Tissue Res. 327(3):499-510).
Western Blotting Analysis: A representative lot detected APN/CD13 in Western Blotting applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in PC3 cell line.
Research Category
Inflammation & Immunology
Quality
Evaluated by Immunohistochemistry in human kidney tissue.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APN/CD13 in human kidney tissue.
Target description
109.54 kDa calculated.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	DF-5, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunocytochemistry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","western blot: suitable
isotype	IgG3
NCBI accession no.	NP_001141,
UniProt accession no.	P15144,
Gene Information	human ... ANPEP(290)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable