Anti-AML1-ETO

Кат. номер

ABC1411-25UL

ABC1411-100UL

Protein CBFA2T1 (UniProt: Q06455; also known as Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2) is encoded by the RUNX1T1 (also known as AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2) gene (Gene ID: 862) in human. In acute myeloid leukemias (AMLs) a recurring chromosomal translocation termed as t(8;21) is reported to generates the AML1 (8:21) ETO leukemia fusion protein that contains the DNA-binding domain of 1 transcription factor RUNX1 and almost all of ETO. It is shown that RUNX1 and the AML1 ETO fusion protein are co-expressed in t(8;21) AML cells and antagonize each other s gene-regulatory functions by co-occupying the binding sites of AML1 ETO activated genes. Both AML1-ETO and the AML1 protein are reported to recognize the same consensus DNA-binding motif (TGT/CGGT). AML1 ETO can either activate or repress transcription of different target genes. It can activate transcription of genes via recruitment of p300 and can repress transcription by competitively displacing RUNX1 and recruiting corepressors such as histone deacetylase 3 (HDAC3). Myeloid leukemia cells with the t(8;21) translocation, such as Kasumi-1 cells, display elevated levels of Bcl-2 that prolongs their life span and contribute to the development of t(8;21) leukemia. (Ref.: Guo, C., et al. (2020). J. Biol. Chem. 295(13) 4212 4223; Klampfer, L., et al. (1996). Proc Natl Acad Sci USA. 93(24); 14059-14064).
Specificity
This rabbit polyclonal antibody detects ETO protein and AML-1-ETO fusion protein. It targets an epitope within 17 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human ETO protein fused to AML1.
Anti-AML1-ETO, Cat. No. ABC1411, is a rabbit polyclonal antibody that detects AML1-ETO and is tested for use in ChIP-seq, Immunoprecipitation, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Kasumi-1; t(8;21) cells expressing AML1-ETO.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AML1-ETO in Kasumi-1 ; t(8;21) cells expressing AML1-ETO.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated AML1-ETO in Kasumi-1; t(8;21) cells expressing AML1-ETO (Courtesy of Dr Jinsong Zhang @ Saint Louis University).
Chromatin immunoprecipitation (ChIP): A representative lot detected AML1-ETO in Kasumi-1; t(8;21) cells expressing AML1-ETO (Courtesy of Dr Jinsong Zhang @ Saint Louis University).
ChIP-seq: A representative lot detected AML1-ETO in ChIP-seq applications (Guo, Chun., et. al. (2020). J Biol Chem. 295(13); 4212-4223.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected AML1-ETO in Chromatin Immunoprecipitation applications (Guo, Chun., et. al. (2020). J Biol Chem. 295(13):4212-4223; Steinauer, Nickolas., et. al. (2019). Blood Adv. 3(9): 1379-1393).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	purified antibody","serum
antibody product type	primary antibodies
clone	polyclonal
mol wt	calculated mol wt 67.57 and 48.74 kDa","observed mol wt ~62 and 100 kDa
purified by	(Unpurified)
species reactivity	human
species reactivity (predicted by homology)	rat, mouse
packaging	antibody small pack of 100 µL
application(s)	ChIP: suitable","western blot: suitable
isotype	IgG
epitope sequence	C-terminal
conjugate	unconjugated
Protein ID accession no.	NP_001185555,
UniProt accession no.	Q06455,
shipped in	dry ice
Gene Information	human ... RUNX1T1(862)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable