Anti-Akt/PKB Antibody, PH Domain, clone SKB1

eCl@ss:

32160702

Кат. номер

5-591

5-591-25UG

5-591-100UG

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.
Quality
routinely evaluated by immunoblot on RIPA lysates from A431 cells
Target description
60 kDa
Physical form
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.
Format: Purified
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	100
biological source	mouse
antibody form	affinity purified immunoglobulin
antibody product type	primary antibodies
clone	SKB1, monoclonal
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µg
Торговая марка	Upstate®
application(s)	flow cytometry: suitable","immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NM_005163,
UniProt accession no.	P31749,
shipped in	dry ice