Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat A0168-1ML

MDL number:	MFCD00162629
NACRES:	NA.46

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.
purified mouse IgG Fc fragment
Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in

• western blot
• enzyme linked immunosorbent assay (ELISA) and
• indirect immunoperoxidase assay (IIPA)

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	goat
antibody form	affinity isolated antibody
antibody product type	secondary antibodies
clone	polyclonal
species reactivity	mouse
application(s)	direct ELISA: 1:50,000, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable