Anti-GFP 11814460001

Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27kDa protein originally isolated from the jellyfish Aequorea victoria. The molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since, GFP requires no additional substrates or cofactors, GFPs green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported.
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.
Specificity
Subtype: Both clones are Mouse IgG1
Monoclonal antibody for detection of both wild-type and mutant forms of GFP or GFP fusions using:

• Immunoprecipitation
• Western blots
• Immunostaining

Features and Benefits
Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200µg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Quality
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:

• Western blot: 1:1000 dilution
• Immunoprecipitation: 2 to 10 µg

Storage conditions (working solution): -15 to -25 °C
Reconstitution
Add 500 µl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.
For life science research only. Not for use in diagnostic procedures.
This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	13.1, monoclonal, 7.1, monoclonal
assay	>90% (HPLC)
form	lyophilized
packaging	pkg of 200 µg
Торговая марка	Roche
isotype	IgG1
conjugate	unconjugated
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash