Aminopeptidase N, Rat

Enzyme Commission (EC) Number:

3.4.11.2 ( BRENDA | IUBMB )

Кат. номер

164599-10U

164599-10UCN

Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that functions to remove N-terminal amino acids from peptides with broad specificity. Aminopeptidase N is believed to play a role in angiogenesis and elevated levels of aminopeptidase N have been reported in patients with various types of cancer and have been correlated with tumor load. Also involved in the final stages of digestion of small peptides in the intestinal brush border.
Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis.
Packaging
10 u in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2
Physical form
In 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
Bhagwat, S.V., et al. 2003. Blood101, 1818.
van Hensbergen, Y., et al. 2002. Clin. Cancer Res.8, 3747.
Ishii, K., et al. 2001. Biol. Pharm. Bull.24, 226.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	= -70C
assay	90% (SDS-PAGE)
Quality Level	100,
form	liquid
specific activity	100 units/mg protein
Торговая марка	Calbiochem®
storage condition	OK to freeze, avoid repeated freeze/thaw cycles
shipped in	dry ice
storage temp.	70°C

WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable