JumpStart™ Taq ReadyMix™

MDL number:	MFCD00166026
NACRES:	NA.55

Кат. номер

P2893-20RXN

P2893-125ML-KC

P2893-100RXN

P2893-125ML

P2893-400RXN

P2893-50ML

P2893-30ML

P2893-100RXN-KC

P2893-275UL-KC

P2893-20RXN-KC

P2893-400RXN-KC

P2893-50ML-KC

P2893-30ML-KC

P2893-2750UL

P2893-275UL

P2893-2750UL-KC

P2893-10ML

JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains JumpStart™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart™ Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
JumpStart™ Taq ReadyMix has been used in quantitative polymerase chain reaction (PCR). It has also been used to identify fibroblast cell surface adenosine receptor (AR) types under hypoxia. It is also suitable:

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers

Features and Benefits

• The master mix allows consistency from one reaction to the next
• Reduced preparation time and reduced risk of contamination from multiple pipetting steps
• Designed to minimize non-specific amplification and contamination
• Increased specificity and target yield
• Reduced primer dimers
• Reduced set-up time as compared to manual or wax Hot Start methods
• Allows for room temperature set-up
• Ideal for high throughput, quantitative PCR applications

• Reduce set-up time, eliminate contamination concerns, and avoid long reactivation steps with JumpStart Readymixes
• JumpStart Taq antibody allows for room temperature set up of reactions making it ideal for high throughput applications
• Prevents amplification of non-specific products and primer dimers, increasing target yields

Packaging
Default reaction volume is 50 µL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
JumpStart Taq ReadyMix is a prepared solution combining the performance benefits of hot start PCR with the convenience of a ReadyMix. The mix includes JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and buffer in a 2x optimized reaction concentrate. JumpStart Taq Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq polymerase does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The hot start mechanism allows for room temperature set up, making it ideal for high throughput applications. To prepare a 50 μL reaction, simply add 25 μL of ReadyMix to template, primers and water for a final reaction volume of 50 μL.
To prepare a 50 µL reaction, simply add 25 µL of ReadyMix™ to template, primers and water for a final reaction volume of 50 µL.
This product has been validated in quantitative PCR, but may require supplementation with magnesium chloride solution, 25 mM, Catalog Number M8787, a suitable fluorescent probe, and, if desired, an internal reference dye, Catalog Number R4526. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
form	liquid
feature	hotstart
concentration	2.5 units/reaction (50 µL reaction volume)
color	colorless
Featured Industry	Agriculture
shipped in	wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable