dTTP

CAS Number:	18423-43-3
MDL number:	MFCD00080338
PubChem Substance ID:	329798929

Кат. номер

11934546001

11969048001

3732711001

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).
dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.
Quality
Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Physical form
solution of dTTP in water (pH 8.3).
Preparation Note
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 µl nucleotide mix add 10 µl of dATP, dCTP, dGTP, dTTP (each) to 60 µl Water, PCR Grade).
For general laboratory use.

Quality Level	100,
assay	<0.9% (dNDP), 99% (dNTP)
form	solution
usage	sufficient for 125,000 standard reactions (03732711001), sufficient for 31,250 standard reactions (11969048001), sufficient for 6,250 standard reactions (11934546001)
packaging	pkg of 1,250 μL (11969048001 [100 mM]), pkg of 4 x 1,250 μL (03732711001 [4x100 mM]), pkg of 250 μL (11934546001 [100 mM])
Торговая марка	Roche
concentration	100 mM
shipped in	dry ice
storage temp.	−20°C
SMILES string	[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
InChI	1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key	KAELIXTVSANDSE-UHFFFAOYSA-N

RIDADR

NONH for all modes of transport