Biotin-16-dUTP 11093070910

Empirical Formula (Hill Notation):	C32H52N7O18P3S
CAS Number:	136632-31-0
Molecular Weight:	947.78

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.
For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.

• Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
• DNA polymerase I (holoenzyme and Klenow fragment)
• Taq DNA polymerase
• Reverse transcriptase (e.g., Transcriptor)

Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
The nucleotide also serves as a substrate for Terminal Transferase in 3-end labeling.

• Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
• Biotin Luminescence Detection Kit

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.
Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100
packaging	pkg of 50 μL (50 nmol; 1mM)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash