RNA Sample Loading Buffer

MDL number:	MFCD00213041
NACRES:	NA.25

Кат. номер

R4268-1ML

R4268-PH

R4268-5VL

R4268-1VL

R4268-1ML-KC

RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.
RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.
Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.
RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot.
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25x working concentration.
RNA loading buffer contains 62.5% deionized formamide, 1.14M formaldehyde, 200 μg/ml bromphenol blue, 200 μg/ml xylene cyanole, and 50 μg/ml ehtidium bromide in MOPS-EDTA-sodium acetate at 1.25x working concentration.
Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.

Quality Level	200
grade	for molecular biology
form	liquid
concentration	1.25 x
application(s)	electrophoresis: suitable
foreign activity	RNase, none detected
storage temp.	20°C, 20°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H317 - H332 - H341 - H350 - H360D - H373
pcodes	P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
Target Organs	Blood
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable