RNase A

Enzyme Commission (EC) Number:

3.1.27.5 ( BRENDA | IUBMB )

Кат. номер

10109169001

10109142001

Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.

• For analytical purposes
• Isolation of DNA (for this purpose, RNase A should be boiled)
• For cell cycle analysis by flow cytometry and propidium iodide (PI) staining

RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
form	dry powder
specific activity	50 U/mg (+25°C, RNA as substrate)
mol wt	Mr = 13,700
packaging	pkg of 100 mg (10109169001), pkg of 25 mg (10109142001)
Торговая марка	Roche
parameter	65 °C optimum reaction temp.
optimum pH	7.0-7.5
shipped in	wet ice
storage temp.	2-8°C

RIDADR

NONH for all modes of transport