GenomePlex® WGA Reamplification Kit WGA3-50RXN

NACRES:

NA.55

GenomePlex WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (µBm-probe and µBsm-probe) labeled with digoxigenin-11-dUTP.
Features and Benefits

• Starting material: 10 ng
• Expected yield: 5 - 10 µg
• Time required: ~1.5 hours
• Provides a method to accurately amplify whole genome amplified DNA
• Successive reamplification with WGA-3 provides DNA with little genetic bias when compared to the original genome
• Maintains representation of the entire genome through subsequent reamplifications
• Preserves precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500-fold)
• Minimal allele drop out maintained through five successive cycles
• Increases the number of downstream assays and allows more information to be gathered on that sample
• Provides the opportunity to re-amplify the WGA DNA without having to purchase an entire WGA kit

The sequences of the universal primers provided in this kit are considered proprietary.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchasers own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchasers activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Quality Level	200,
application(s)	whole genome amplification: suitable
shipped in	wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable