Lipopolysaccharides from Escherichia coli O26:B6

EC Number:	297-473-0
MDL number:	MFCD00164401

Кат. номер

L8274-100MG-PW

L8274-10MG-PW

L8274-100MG

L8274-10MG

L8274-25MG

L8274-25MG-PW

This product is phenol extract from E. coli serotype O26:B6. The source strain is ATCC 12795. LPS from serotype 026:B6 is the only LPS from E. coli which exhibits short chain-length behavior on SDS-PAGE. The short chain-length of this LPS is closer to that of the mutant rough strain LPS. This LPS serotype has been used at 1 ug/ml to stimulate neutrophils.
10 mg in poly bottle
25, 100 mg in glass bottle
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

biological source	Escherichia coli (O26:B6)
Quality Level	200
form	lyophilized powder
specific activity	10,000 EU/mg
purified by	phenol extraction
impurities	<3% Protein (Lowry)
shipped in	ambient
storage temp.	2-8°C

pictograms	GHS06
signalword	Danger
hcodes	H300
pcodes	P264 - P270 - P301 + P310 - P405 - P501
Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable