THP 1 Cell Line human

Кат. номер

88081201-DNA-5UG

88081201-1VL

88081201-CDNA-20UL

88081201-RNA-5UG

Human monocytic leukaemia
Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for -napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Please note: We routinely resuscitate and culture this cell line with success, however, some laboratories have found this cell line difficult to establish following resuscitation from a frozen ampoule. Our Quality Control (QC) Department strongly recommend that this line is supplied as a Growing culture whenever possible e.g. to locations within or near the European Union. We routinely resuscitate and maintain this cell line growing in our laboratory to enable us to supply growing cultures quickly. If however, you still wish to receive the cell line Frozen we can supply but our normal replacement Policy will not apply should you encounter resuscitation difficulties.We recommend, if you are in the European Union (where we can deliver growing cultures), you select a Growing culture.
THP 1 cell line human has been used to study:

• bactericidal activity of moxifloxacin against Staphylococcus aureus
• the effect of lipopolysaccharide-mediated inflammatory response in murine versus human cell lines
• the effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum
• the change in gene expression after infection with Mycobacterium tuberculosis
• the induction of cytokines in the cell line in presence of antigens obtained from Clostridium difficile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16
RPMI 1640, + 2mM Glutamine, + 10% Foetal Bovine Serum (FBS / FCS),.
If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% fetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human blood
growth mode	Suspension
karyotype	Not specified
morphology	Monocyte
products	Not specified
receptors	Fc, C3b
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

RIDADR	NONH for all modes of transport
WGK Germany	3