SK-PN-DW

Кат. номер

94092301-DNA-5UG

94092301-CDNA-20UL

94092301-RNA-5UG

94092301-1VL

Human primitive neuroectodermal tumour
SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.
Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an in vitro system for investigation of the post translational processing of the cck preprohormone.
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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biological source	human nerve
growth mode	Semi-adherent
karyotype	82XXY
morphology	Polygonal
products	Pro-cholecystokinin (Pro-cck)
receptors	Vimentin, P53, anti-MIC2
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

RIDADR

NONH for all modes of transport