MTSV1-7 CE1

Кат. номер

10081202-DNA-5UG

10081202-CDNA-20UL

10081202-1VL

10081202-RNA-5UG

Breast cancer, morphogenesis, adhesion properties
MTSV1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 transfected with ERBB2 expression vector, enabling the over-expression of ERBB2 proto-oncogene. Cultures of MTSV1-7 were transfected with the pSV2-erbB2 construct together with the pSV2neo plasmid. (See also 1-7HB2 Sigma catalog number 10081201).
The MTSV1-7 CE1 cell line is an ideal tool for investigation of the role of the ERBB2 receptor proto-oncogene in breast cancer morphogenesis and adhesion. MTSV1-7 CE1 cells over-express the ERBB2 receptor, inducing a reduced ability to undergo morphogenesis in vitro, and reduced expression of surface adhesion molecules E-Cadherin and alpha-2 integrin. Inhibition of morphogenesis and transcription of adhesion molecules in human mammary epithelial cells can be affected by signals generated by the ERBB2 receptor, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.
STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human breast
growth mode	Adherent
karyotype	Not specified
morphology	Cuboidal morphology at confluence
products	Formation of disorganised structures in collagen type I gels.
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3