JY human cell line

Кат. номер

94022533-DNA-5UG

94022533-CDNA-20UL

94022533-RNA-5UG

94022533-1VL

Homozygous: No
Consanguineous: No
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
A: 201
Cw: 702
B: 702
HLA DQA1: 3011
HLA DPB1: 201
HLA A: 2
HLA C: 7
HLA B: 7
Bw4/bw6: 6
HLA DR: 4 13
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3
RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
When resuscitating from frozen, cells should be seeded at 0.5 x 106 cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 105 cells/ml. Maintain cultures between 3-9 x 105 cells/ml for maximum growth; 5% CO2; 37°C. Cells form floating aggregates of varying size.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Amish
gender	male
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture | mammalian: suitable
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3