1.2B4

Кат. номер

10070103-CDNA-20UL

10070103-RNA-5UG

10070103-DNA-5UG

10070103-1VL

1.2B4 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line (Sigma catalog number 93121055). 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 10,13
D5S818: 10,14
D7S820: 12
THO1: 9
TPOX: 8,12
vWA: 18
RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS
Split sub-confluent cultures (70-80%) 1:2 to 1:6 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.
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Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human pancreas (islets)
growth mode	Adherent
karyotype	Modal chromosome number 67-71
morphology	Epitheloid
products	1.2B4 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter.
receptors	Not specified
application(s)	cell culture | mammalian: suitable
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3