HiLoad® 16/600 Superdex® 200 pg GE28-9893-35
HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants
Store at 4 to 30 °C (20% Ethanol)
Discover LiChropur reagents ideal for
HPLC, or
LC-MS, analysis
HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva
| material | bed support (polyamide), glass column, glass fiber (polypropylene reinforced) |
| shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| mol wt | 10000-600000 Da (globular proteins) |
| packaging | pkg of 1 ea |
| Торговая марка | Cytiva |
| parameter | <1.0 mL/min flow rate, 0.3 Mpa (42 psi) |
| bed volume | 120 mL |
| column L x I.D. | 60 cm x 16 mm |
| sample volume | <5 mL |
| matrix | agarose dextran composite, dextran-agarose particle platform, polymeric |
| matrix active group | dextran-agarose phase |
| avg. part. size | 34 µm |
| working range | 3-12 |
| cleaning | 1 - 14 |
| separation technique | size exclusion (SEC) |
| RIDADR | NONH for all modes of transport |