Anti-TFE3 Antibody, clone 1G5 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1272-4X25UL

ZRB1272-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1G5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Transcription Factor E3 (TFE3). It targets an epitope within the C-terminal half.
His-tagged recombinant fragment corresponding to 143 amino acids from the C-terminal half of human Transcription factor E3 (TFE3).
Anti-TFE3, clone 1G5 ZooMAb®, Cat. No. ZRB1272, is a recombinant Rabbit monoclonal antibody that specifically targets TFE3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFE3 in NIH3T3 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TFE3 in HeLa and C6 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Recombinant TFE3 Protein with a KD of 1.6 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TFE3 in human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TFE3 in PC3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Transcription factor E3 (UniProt: P19532; also known as Class E basic helix-loop-helix protein 33, bHLHe33, TFE3) is encoded by the TFE3 (also known as BHLHE33) gene (Gene ID: 7030) in human. TFE3 is a ubiquitously expressed transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. It specifically recognizes and binds E-box sequences (5-CANNTG-3) and its efficient DNA-binding requires homodimerization or heterodimerization with another member of the MiT/TFE family, such as TFEB or MITF. TFE3 is reported to be involved in the cellular response to amino acid availability by acting downstream of MTOR. In the presence of nutrients, it undergoes phosphorylation by mTOR that promotes its cytosolic retention and subsequent inactivation. However, under conditions of starvation or lysosomal stress, inhibition of mTOR induces TFE3 dephosphorylation that results in its nuclear localization and transcription factor activity. TFE3 is also reported to act as a positive regulator of browning of adipose tissue by promoting expression of target genes. It also plays a role in maintenance of the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB. Its mTOR-dependent nuclear exclusion promotes exit of cells from pluripotency. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1G5, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 61.52 kDa, observed mol wt ~ 59 kDa
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable