Anti-Prion Protein Antibody, clone 3D17 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1268-25UL

ZRB1268-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3D17 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Prion protein. It targets an epitope within 19 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Prion protein.
Anti-Prion Protein, clone 3D17 ZooMAb®, Cat. No. ZRB1268, is a recombinant Rabbit monoclonal antibody that specifically targets Prion Protein and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Prion Protein in Human brain tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Prion Protein in human cerebral cortex and human Alzheimers diseased brain tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Prion peptides with a KD of 8.4 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prion Protein in SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Major prion protein (UniProt: P04156; also known as PrP, ASCR, PrP27-30, PrP33-35C, CD230) is encoded by the PRNP (also known as ALTPRP, PRIP, PRP) gene (Gene ID: 5621) in human. PrP is highly expressed in the brain, lung, kidney and heart and low levels of expression are reported in the liver and spleen. It is found in high quantity in the brain of humans and animals infected with degenerative neurological diseases such as Kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), scrapie, bovine spongiform encephalopathy (BSE), and transmissible mink encephalopathy (TME). In normal brain it may play a role in neuronal development and synaptic plasticity and may be required for neuronal myelin sheath maintenance and myelin homeostasis through acting as an agonist for ADGRG6 receptor. PrP can exist in either PrPc (c = cellular) or PrPsc (sc = Scrapie) form. PrPc is a glycoprotein normally found inserted in the plasma membrane by a glycosyl phosphatidylinositol (GPI) anchor and can be easily digested by proteases. It is composed mainly of -helices. The PrPsc has more of a -sheet type structure and is highly resistant to the action of proteases. The prion proteins possess two N-linked glycosylation sites and may contain over 50 different sugars. The large size of the N-linked sugars enables them to shield two orthogonal faces of the protein almost completely and protect large regions of the protein surface from proteases. The PrPsc is shown to contain the same set of glycans as PrPc but has a higher proportion of tri- and tetra-antennary sugars. It is shown that following proteinase K treatment, PrPc is completely digested, whereas PrPsc is only shortened to a 27-30 kDa fragment. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3D17, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 27.66 kDa, observed mol wt ~ 35 kDa
species reactivity	mouse, human
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable