ADP/ATP Ratio Assay Kit MAK135-1KT

NACRES:

NA.84

Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP are much more pronounced in necrosis versus apoptosis.
Compatible with high-throughput handling systems.
Suitable for the detection of apoptosis and necrosis in cells and for the studying the effects of compounds on cellular proliferation.
The ADP/ATP Ratio Assay kit provides a simple and direct procedure for measuring ADP and ATP levels in cells for the screening of apoptosis, necrosis, and cell proliferation. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of the intracellular ATP concentration.
Luciferase
ATP + D-Luciferin + O2 ----------> oxyluciferin + AMP + PPi + CO2 + light
In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.

usage	sufficient for 100 tests (bioluminescent)
detection method	chemiluminescent
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317 - H412
pcodes	P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
RIDADR	NONH for all modes of transport