Rapid Cell Proliferation Kit

Кат. номер

QIA127-1KIT

QIA127-1KITCN

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
1 kit in Glass bottle
Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)
WST-1, 1-Methoxy PMS, and a user protocol.
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Assay Time: 0.5 - 4 h
The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.
Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	-20C
Quality Level	100,
usage	sufficient for 500 tests
packaging	pkg of 1 96-well plate(s)
Торговая марка	Calbiochem®
storage condition	OK to freeze, avoid repeated freeze/thaw cycles, protect from light
input	sample type cultured cells
test parameters	sample: 1000 - 50,000 cells
detection method	colorimetric
shipped in	wet ice
storage temp.	20°C