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TM3
Кат. номер 91060526-DNA-5UG 91060526-1VL 91060526-RNA-5UG Cell Line OriginMouse BALB/c testis, Leydig cellCell Line DescriptionOriginated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.ApplicationHormone studiesCulture MediumHam′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse testis growth mode Adherent karyotype Not specified morphology Not specified products Prostaglandin F2a receptors Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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TM4
Кат. номер 88111401-DNA-5UG 88111401-1VL Cell Line OriginMouse BALB/c testis sertoli cellCell Line DescriptionFrom primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.ApplicationHormone studiesCulture MediumHam′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse testis growth mode Adherent karyotype Not specified morphology Not specified products Transferrin, H-Y Ag, novel retinol-binding protein receptors Androgen, oestrogen and progesterone receptors application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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TO human cell line
Кат. номер 94022549-CDNA-20UL 94022549-RNA-5UG 94022549-DNA-5UG 94022549-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 6802 3001
Cw: 0602 15
B: 1302 5101Serological ProfileHLA A: 68 30
HLA A ief: 68.2
HLA C: 6
HLA B: 13 51
Bw4/bw6: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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TO14
Кат. номер 10081203-1VL 10081203-RNA-5UG 10081203-CDNA-20UL 10081203-DNA-5UG Cell Line OriginHuman ovarian cancer; oestrogen receptor negative, drug efficacy and toxicityCell Line DescriptionTO14 is an adherent cell line derived from a metastasis to the omentum obtained at hysterectomy from a patient with a well differentiated serous adenocarcinoma. TO14 exhibits poor growth in semi-solid medium (agar). TO14 is from the same patient as PEO23 and PEO14 cell lines (Sigma catalog numbers 10032313 and 10032311 respectively).
This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) available at ECACC which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists.DNA ProfileNot specifiedCulture MediumRPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2<sup/>Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human ovary growth mode Adherent karyotype For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72. morphology Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres. products Oestrogen receptor negative cell line. receptors Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72. application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice
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TOKUNAGA human cell line
Кат. номер 85102916-1VL 85102916-DNA-5UG 85102916-CDNA-20UL 85102916-RNA-5UG General descriptionHomozygous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 2402
HLA DRB1: 1502
HLA DRB5: 102
HLA DRB6: 2
HLA DQA1: 103
HLA DQB1: 601
HLA DPA1: 2
HLA DPB1: 901Serological ProfileHLA A: 24
HLA B: 52
Bw4/bw6: 4
HLA Dw: 12
HLA DR: 15
HLA DR51: 51
HLA DQ: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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TOU (TOU I-2)
Кат. номер 93093001-DNA-5UG 93093001-CDNA-20UL 93093001-1VL 93093001-RNA-5UG Cell Line OriginHuman B lymphocyte, EBV transformedCell Line DescriptionPeripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion II (del EP1-A1-GP) Gm-Am genotype Gm b; -; -A2m2/Gm g; za; .A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). Only one allotype is defined on the gamma 2 chain and may or may not be present; (.) indicates the absence of this allotype.Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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TPS
Кат. номер 96112839-RNA-5UG 96112839-1VL 96112839-DNA-5UG Cell Line OriginFish Rainbow Trout Pronephric StromaCell Line DescriptionTPS has been initiated from a long-term pronephric stroma culture of an adult 1 year old rainbow trout (Oncorhynchus mykiss W). The cells grow at 4° to 22°C, with an optimum temperature of 18°C. They do not form true monolayers, but overlap in a multilayered network of fibroblastic, epithelioid and giant cells. TPS cells are susceptible to viral haemorrhagic septicemia virus (VHSV) and infectious haemopoietic necrosis virus (IHNV). They have been applied to support the development of haemopoiesis when co-cultured with haemopoietic precursors. Spontaneous angiogenesis processes in vitro occurred in TPS cultures when they are kept in the same flask, i.e. without passages, for long times (over 2 months).ApplicationFish Haemopoiesis and Fish pathology.Culture MediumRPMI 1640 + 2mM Glutamine + 25mM Hepes + 2.5mM NaP + 0.01mM Hydrocortisone-21-hemisuccinate + 0.088mM Guanosine + 0.0225mM Cytidine + 0.094mM Adenosine + 0.102mM Uridine + 10% FBS + 10% HS, pH 7.4-7.6. At ECACC successful growth of this cell line has beeSubculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; trypsinise at room temperature once a week; air atmosphere; 18°C. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fish kidney (anterior) growth mode Adherent karyotype Modal no. 58 morphology Fibroblast-like/Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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TR146 cell line human 10032305-1VL
General descriptionThe TR146 cells are appropriate for drug transport studies and they mimic normal human buccal epithelium. It serves as an oral mucosa model for barrier property studies with various molecules including tight junction proteins, aquaporins and epithelial-mesenchymal transition (EMT) markers.Cell Line OriginHuman squamous cell carcinomaCell Line DescriptionTR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.ApplicationTR146 cell line human has been used:- in cytotoxicity studies with an antibacterial compound, metronidazole
- in viability and metabolic studies post blue laser light exposure,
- as an oral epithelium-resembling cell line model for in vitro C. albicans infection studies
- in barrier function studies using transepithelial electrical resistance (TEER)
- for the transport studies with C-reactive protein (CRP)
DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18Culture MediumHAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density of 5 x 104 cells/cm2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.ПараметрыQuality Level 100, biological source human mouth description Human squamous cell carcinoma growth mode Adherent karyotype Not specified morphology Polygonal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TR33B
Кат. номер 85040102-RNA-5UG 85040102-DNA-5UG 85040102-1VL Cell Line OriginRat Wistar-Furth oligodendrogliomaCell Line DescriptionEthyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (Sigma Catalogue number. 85081901).ApplicationTumourigenicity studiesCulture MediumDMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Adherent karyotype Not specified morphology Glial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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TR6Bc1
Кат. номер 85042301-DNA-5UG 85042301-RNA-5UG 85042301-1VL Cell Line OriginMouse C3H.He SchwannomaCell Line DescriptionEthyl nitrosourea induced tumour in C3H. The mice, originated in trigeminal nerve and brain; readily transplanted and resembles schwannoma histologically. Cultured cells form prominent processes, resemble 33B (Sigma Catalogue number. 85081901) morphologically, contain abundant C type particles.ApplicationTumourigenicity studiesCulture MediumDMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Glial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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TRA-171
Кат. номер 90120514-1VL 90120514-RNA-5UG 90120514-DNA-5UG Cell Line OriginInsect larvae, mosquitoCell Line DescriptionThe cell line TRA-171 has been established from freshly hatched larvae of Toxorhynchites amboinensis. After eggs had been surface-sterilised, they were allowed to hatch in MM / VP12 medium and were mechanically damaged at the anterior or posterior ends of the body in 0.25% trypsin. After washing and cultivation at 22 - 25°C small patches of cells were visible leading to the isolation of the cell line TRA-171 after 72 days in culture. The cells have been adapted to grow in a 1:1 mixture of Leibovitz L-15 and Mitsuhashi and Maramorosch media plus supplements. Four serotypes of laboratory adapted and three type strains (1, 2 and 3) of un-adapted dengue virus will replicate in the cells at 28°C.ApplicationVirus studies: Dengue virusCulture MediumL15: Mitsuhashi and Maramorosch Insect Medium (1:1) + 2mM Glutamine + 0.05% BSA + 1% NEAA + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin for 5 minutes only at 28°C, after quenching the trypsin with fresh medium dislodge cells by gentle scraping; CO2 not required; 28°C. FrOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source insect larvae growth mode Adherent karyotype Not specified morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Trex1 KO Cell line from mouse
Кат. номер 12021509-1VL 12021509-RNA-5UG 12021509-DNA-5UG Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source mouse embryo growth mode adherent morphology fibroblast shipped in dry ice storage temp. −196°C
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tsa201 Cell Line human
Кат. номер 96121229-RNA-5UG 96121229-1VL 96121229-CDNA-20UL Cell Line OriginHuman embryonal kidney, SV40 transformedCell Line DescriptiontsA201 is a transformed human kidney (293, Sigma catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.Applicationtsa201 human cell line has been used for protein-binding studies, whole-cell patch clamp recordings and transfection studies.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:8; cells are dislodged by mechanical agitation or EDTA solution; 5% CO2; 37°C. Do not let cells become over confluent as this can affect morphology.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human kidney growth mode Adherent karyotype Not specified morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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tsDC
Кат. номер 1081609-RNA-5UG 1081609-DNA-5UG 1081609-1VL Cell Line OriginImmortalised mouse dendritic cell lineDNA ProfileNot specifiedCulture MediumIDMEM (I3390), + 2 mM L-Glutamine (G7513), + 5% FBS (F2442), + 100U/ml Penicillin + 100 ug/ml Streptomycin + 0.05 mM 2ME (M6250),Subculture RoutinePassage cells approximately once every 2 weeks. Culture at 33 °C; 5-10% CO2. Cells are of heterogenous morphology, but the line has been cloned. Some cells adhere and others grow in suspesion. Cells are detached by short incubation in PBS plus 20mM EDTA, followed by washing in culture medium. Despite the cells slow growth, they can be highly diluted -growth does not depend on a minmum cell density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationThis material is cited in US and/or other patent and may not be used to infringe patent claims. US patent No′s. 5688692, 5866759 and international patent pending PCT/GB91/00262.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source mouse bone growth mode Semi-adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
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TT
Кат. номер 92050721-RNA-5UG 92050721-1VL 92050721-DNA-5UG 92050721-CDNA-20UL Cell Line OriginHuman Caucasian thyroid medullary carcinomaCell Line DescriptionEstablished from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.ApplicationTumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18Culture MediumHam′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommenOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human thyroid growth mode Adherent karyotype Aneuploid karyotype with marker chromosomes morphology Not specified products Calcitonin, Carcinoembryonic antigen (CEA) receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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TUBO human cell line
Кат. номер 88052045-DNA-5UG 88052045-RNA-5UG 88052045-CDNA-20UL 88052045-1VL General descriptionConsanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0216 0301
Cw: 0704 1502
B: 5101
HLA DRA: 0101 0102
HLA DRB1: 1104 1201
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 02012 0402Serological ProfileHLA A: 2 3
HLA A ief: 2.3 3.2
HLA C: 7
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA Dw: 5 DB6
HLA DR: 11 12
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity French gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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U-2 OS Cell Line human
Кат. номер 92022711-DNA-5UG 92022711-1VL 92022711-CDNA-20UL 92022711-RNA-5UG Cell Line OriginHuman OsteosarcomaCell Line DescriptionCell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.ApplicationU-2 OS has been used to study:- the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
- the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
- calcium-mediated actin reset (CaAR) in response to physiological changes
DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18Culture MediumMcCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human bone growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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U-251 MG cell line human
Кат. номер 9063001-DNA-5UG 9063001-RNA-5UG 9063001-CDNA-20UL 9063001-1VL Cell Line OriginHuman glioblastoma astrocytomaCell Line DescriptionDerived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma product no. 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (Sigma product no. 08061901).Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under product no. 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′ and has the new Sigma product no. 09063001.ApplicationU-251 MG cell line human has been used:- for the extraction of cancer stem cells from U-251 human glioblastoma cell line
- to study the mechanism of Pax6 (paired box protein)-associated increase in expression of Dkk3 (Dickkopf 3)
- to study the role of JARID1B (jumonji AT-rich interactive domain 1B) in the pathogenesis of glioma
- to study the therapeutic efficacy of Irinophore C combined with temozolomide in glioblastoma tumor model
DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human brain growth mode Adherent karyotype 2n = 46 morphology Pleomorphic/astrocytoid products Contains GFAP positive cells receptors PDGFR alpha and EGFR. See Nistr M. et al., (1988) & (1991) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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U-87 MG Cell Line human
Кат. номер 89081402-1VL 89081402-CDNA-20UL 89081402-RNA-5UG Cell Line OriginHuman glioblastoma astrocytomaCell Line DescriptionDerived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.ApplicationU-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.
Neural studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human brain (glioblastoma astrocytoma) growth mode Adherent karyotype 2n = 46 morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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U-BLC1
Кат. номер 6013102-CDNA-20UL 6013102-1VL 6013102-DNA-5UG 6013102-RNA-5UG Cell Line OriginHuman bladder transitional cell carcinomaCell Line DescriptionSpontaneously immortal cell line established from a primary transitional cell carcinoma of the bladder. Derived from a female patient.Culture Medium2/3 DMEM:1/3 RPMI + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The medium must be changed to fresh medium every 48 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human bladder growth mode Not specified karyotype Aneuploid, with specific marker chromosomes morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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U266B1
Кат. номер 85051003-RNA-5UG 85051003-CDNA-20UL 85051003-DNA-5UG 85051003-1VL Cell Line OriginHuman myelomaCell Line DescriptionThis cell line was derived from peripheral blood of a patient with an IgE myeloma (epsilon2,lambda2). The line has been reported to secrete IgE and is used as fusion partner for the production of hybridomas. U266B1 has been reported to produce interleukin-6 (IL-6).ApplicationFusion partnerDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Lymphoblast products IgE receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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U28
Кат. номер 93022518-DNA-5UG 93022518-RNA-5UG 93022518-1VL Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe hybridoma U28 was obtained by fusing SP2/0 myeloma cells with spleen cells from BALB/c mice that had been given repeated intranasal infection with a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" allotype and the Fc region. There is no cross reactivity with A/PR/8/34 influenza virus, DNP, NP or DNA cytoskeletal proteins. Contains a CH2 domain.ImmunogenA/PR/8/34 mouse pathogenic human influenza virusApplicationDifferential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantificationCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-IgG2a, mouse application(s) cell culture | mammalian: suitable isotype IgM shipped in dry ice storage temp. −196°C
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U2A cell line human
Кат. номер 12021502-DNA-5UG 12021502-RNA-5UG 12021502-1VL 12021502-CDNA-20UL General descriptionFor complete product information, please see ECACC,Cell Line DescriptionThe human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U2A mutant cell line lacks expression of p48 gene product. U2A is unresponsive to interferon-alpha and interferon-beta; furthermore it is partially defective in response to interferon-gamma.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human fibrosarcoma growth mode Adherent morphology Epithelial shipped in dry ice storage temp. −196°C
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U3A Cell line human
Кат. номер 12021503-CDNA-20UL 12021503-DNA-5UG 12021503-RNA-5UG 12021503-1VL Cell Line DescriptionThe human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U3A mutant cell line lacks expression of STAT1 gene product. U3A is unresponsive to interferon-alpha and interferon-gamma. U3A is partially defective in its response to double stranded RNA.DNA ProfileSTR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19Culture MediumDMEM (D6546), + 2mM L-Glutamine (G7513), + 10% FBS (F2442),.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.CitationIf use of this culture results in a scientific publication, it should be cited in the publication as: U3A (ECACC 12021503)DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human fibrosarcoma morphology Epithelial shipped in dry ice storage temp. −196°C
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U4A Cell line human
Кат. номер 12021504-1VL 12021504-RNA-5UG 12021504-CDNA-20UL 12021504-DNA-5UG Culture MediumDMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human fibrosarcoma growth mode Adherent morphology Epithelial shipped in dry ice storage temp. −196°C
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U4C cell line human
Кат. номер 12021505-RNA-5UG 12021505-1VL 12021505-DNA-5UG 12021505-CDNA-20UL Cell Line DescriptionThe human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U4C mutant lacks the JAK1 kinase. U4C is unresponsive to interferon alpha and interferon gamma; furthermore it is partially defective in response to double stranded RNA.DNA ProfileSTR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19Culture MediumDMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human fibrosarcoma growth mode Adherent morphology Epithelial shipped in dry ice storage temp. −196°C
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U5A Cell line human
Кат. номер 12021506-DNA-5UG 12021506-CDNA-20UL 12021506-1VL 12021506-RNA-5UG Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human fibrosarcoma growth mode Adherent morphology Epithelial shipped in dry ice storage temp. −196°C
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U6A Cell line human
Кат. номер 12021507-DNA-5UG 12021507-RNA-5UG 12021507-CDNA-20UL 12021507-1VL Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human fibrosarcoma growth mode adherent morphology epithelial shipped in dry ice storage temp. −196°C
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U937 Cell Line human
Кат. номер 85011440-RNA-5UG 85011440-1VL 85011440-DNA-5UG 85011440-CDNA-20UL Cell Line OriginHuman Caucasian histiocytic lymphomaCell Line DescriptionDerived from malignant cells of a pleural effusion of 37 year old caucasian male with diffuse histiocytic lymphoma. One of only a few human lines still expressing many of the monocytic like characteristics exhibited by cells of histiocytic origin.ApplicationU937 Cell Line human has been used to study the toxicity of propolis extracts against U937 cells and to study the uptake of apoptotic bodies (derived from monocytic U-937 cells) by primary meningothelial cells (PMECs).
Tumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 14,15Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C. Cells may take up to 72 hours until confluent.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (lymphoblast) growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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UKRV-Mel-14b
Кат. номер 13012529-CDNA-20UL 13012529-1VL 13012529-DNA-5UG 13012529-RNA-5UG Cell Line DescriptionUKRV-Mel-14b (ESTDAB-225) was established from the lymph node metastasis in a 52 year-old female patient with metastatic melanoma (superficial spreading melanoma stage IV). The UKRV-Mel-14b cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB, contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 7,9
TPOX: 8,9
vWA: 15,17Culture MediumRPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 1-3 x 10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 8 x 10,000 cells/cm .Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human lymph node description Human melanoma, metastasis growth mode Adherent morphology Epithelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
UKRV-Mel-17
Кат. номер 13012514-DNA-5UG 13012514-RNA-5UG 13012514-CDNA-20UL 13012514-1VL Cell Line DescriptionUKRV-Mel-17 (ESTDAB-147) was established from the lymph node metastasis in a 48 year-old male patient with metastatic melanoma (skin stage IV). The UKRV-Mel-17 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB, contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.DNA ProfileSTR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 10,13
D5S818: 11,12
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 15,16Culture MediumRPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 1-4 x10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 8 x10,000 cells/cm.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human lymph node description Human melanoma, metastasis growth mode Adherent morphology Epithelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
UKRV-Mel-19b
Кат. номер 13012536-1VL 13012536-RNA-5UG 13012536-DNA-5UG 13012536-CDNA-20UL Cell Line DescriptionUKRV-Mel-19b (ESTDAB-231) was established from the lymph node metastasis in a 53 year-old female patient with metastatic melanoma (unknown primary stage IV). The UKRV-Mel-19b cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB, contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 11,12
THO1: 9
TPOX: 8
vWA: 14,17Culture MediumRPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 1-3 x 10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 20 x 10,000 cells/cm .Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human lymph node description Human melanoma, metastasis growth mode Adherent morphology Epithelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
UKRV-Mel-3
Кат. номер 13012499-RNA-5UG 13012499-1VL 13012499-DNA-5UG 13012499-CDNA-20UL Cell Line DescriptionUKRV-Mel-3 (ESTDAB-176) was established from the cutaneous/subcutaneous metastasis in a 33 year-old female patient with metastatic melanoma (stage IV). The UKRV-Mel-3 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB, contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11,12
D5S818: 12
D7S820: 10,11
THO1: 9
TPOX: 8,11
vWA: 17,19Culture MediumRPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 1-4 x10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 8 x10,000 cells/cm .Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human skin description Human melanoma, metastasis growth mode Adherent morphology Epithelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
UKRV-Mel-6b
Кат. номер 13012538-RNA-5UG 13012538-1VL 13012538-DNA-5UG 13012538-CDNA-20UL Cell Line DescriptionUKRV-Mel-6b (ESTDAB-222) was established from the cutaneous/subcutaneous metastasis in a 51 year-old female patient with metastatic melanoma (primary nodular melanoma stage IV). The UKRV-Mel-6b cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB, contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 12,13
THO1: 6
TPOX: 8
vWA: 18Culture MediumRPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 1-3 x 10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 5 x 10,000 cells/cmOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human skin description Human melanoma, metastasis growth mode Adherent morphology Epithelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
UKTS8049
Кат. номер 91032120-1VL 91032120-RNA-5UG 91032120-CDNA-20UL 91032120-DNA-5UG Cell Line OriginUK Caucasian MaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender male growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8670
Кат. номер 91031441-CDNA-20UL 91031441-DNA-5UG 91031441-RNA-5UG 91031441-1VL Cell Line OriginUK Caucasian MaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender male growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8695
Кат. номер 91021102-CDNA-20UL 91021102-DNA-5UG 91021102-RNA-5UG 91021102-1VL Cell Line OriginUK Caucasian FemaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender female growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8713
Кат. номер 91031446-RNA-5UG 91031446-DNA-5UG 91031446-1VL 91031446-CDNA-20UL Cell Line OriginUK CaucasianCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender not specified growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8727
Кат. номер 91021107-DNA-5UG 91021107-CDNA-20UL 91021107-1VL 91021107-RNA-5UG Cell Line OriginUK CaucasianCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 5 ethnicity UK Caucasian gender not specified growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8740
Кат. номер 91031437-DNA-5UG 91031437-CDNA-20UL 91031437-1VL 91031437-RNA-5UG Cell Line OriginUK Caucasian MaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender male growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8754
Кат. номер 91070908-DNA-5UG 91070908-1VL 91070908-CDNA-20UL 91070908-RNA-5UG Cell Line OriginUK Caucasian FemaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender female growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8768
Кат. номер 91031439-1VL 91031439-DNA-5UG 91031439-RNA-5UG 91031439-CDNA-20UL Cell Line OriginUK Caucasian FemaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender female growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8791
Кат. номер 91032122-CDNA-20UL 91032122-DNA-5UG 91032122-RNA-5UG 91032122-1VL Cell Line OriginUK Caucasian MaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender male growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8792
Кат. номер 91032123-1VL 91032123-DNA-5UG 91032123-RNA-5UG 91032123-CDNA-20UL Cell Line OriginUK Caucasian FemaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender female growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8796
Кат. номер 91032124-1VL 91032124-DNA-5UG 91032124-CDNA-20UL 91032124-RNA-5UG Cell Line OriginUK Caucasian MaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender male growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8797
Кат. номер 91032125-CDNA-20UL 91032125-DNA-5UG 91032125-RNA-5UG 91032125-1VL Cell Line OriginUK Caucasian MaleCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 4 ethnicity UK Caucasian gender male growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8802
Кат. номер 91032126-1VL 91032126-DNA-5UG 91032126-CDNA-20UL 91032126-RNA-5UG Cell Line OriginUK CaucasianCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 5 ethnicity UK Caucasian gender not specified growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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UKTS8825
Кат. номер 91030444-CDNA-20UL 91030444-RNA-5UG 91030444-1VL 91030444-DNA-5UG Cell Line OriginUK CaucasianCell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + 20% FBS / FCS, (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood HRC Panel Number 5 ethnicity UK Caucasian gender not specified growth mode Suspension karyotype Not Specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable
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