Gelatin solution

CAS Number:	9000-70-8
MDL number:	MFCD00081638
NACRES:	NA.75

Кат. номер

G1393-EW

G1393-100ML

G1393-PH-X

G1393-100ML-PW

G1393-20ML-PW

G1393-20ML

Gelatin is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. Gelatin has wide applications in food industry. It takes up random coil structure after digestion from the triple helical collagen. The most common source for industrial production of gelatin is slaughter byproducts. The type A gelatin and type B differ in their isoelectric pH. This product is recommended for use as a cell culture substratum at 1-5 µg/cm2 or 0.5-50 µg/mL. The optimal concentration does depend on cell type as well as the application and research objective.
Gelatin solution has been used:

• for coating of cell culture plates or dishes used for embryonic stem cell culture, testicular cell culture and neural rosettes
• in coating cell culture to improve attachment of cells
• in PCR to stabilize Taq DNA
• as a blocking reagent in western blotting
• in ELISA
• in immunochemistry
• as a component of media for species differentiation in bacteriology
• to study long-chain fatty acid-induced changes in gene expression in neonatal cardiac monocytes
• to study to test mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo

Gelatin has gelling property and displays surface behaviour for use in foams and adhesions. It also serves as a clarification agent in fruit based juices. It is a digestible protein and serves as a binding agent, thickener, stabilizer and emulsifier in food industry. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications.
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.

biological source	bovine hide
Quality Level	200,
sterility	sterile
type	Type B
product line	BioReagent
form	solution
packaging	pkg of 100 mL, pkg of 20 mL
concentration	2% in H2O, tissue culture grade
application(s)	cell culture | mammalian: suitable
surface coverage	100200 µg/cm2
impurities	endotoxin, tested
solubility	water: soluble
shipped in	ambient
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable