| Human Protein Atlas Number: | HPA010537 Human Protein Atlas characterization data |
LRRK1 (leucine-rich repeat kinase 1) belongs to the four member ROCO protein superfamily, and shares high homology with LRRK2. This protein contains the characteristic Ras-related GTPase or Roc domain, a COR (C-terminal Of Roc) motif and a kinase domain. It is an orphan protein with a molecular weight of 200kDa, and is expressed in brain.
Leucine-rich repeat serine/threonine-protein kinase 1 recombinant protein epitope signature tag (PrEST)
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org),and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols, and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige,.
Biochem/physiol Actions
In LRRK2-associated Parkinson patients, the interaction of LRRK1 (leucine-rich repeat kinase 1) with LRRK2 might determine the age at which Parkinson disease (PD) symptoms manifest themselves. This protein is responsible for the trafficking of EGFR (epidermal growth factor receptor) from early to late endosomes. This is dependent upon EGFR-mediated phosphorylation of LRRK1 protein, which functions as a feedback mechanism. The expressions of both LRRK1 and LRRK2 are developmentally regulated, and their functions might overlap.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Corresponding Antigen APREST71435,.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| form | buffered aqueous glycerol solution |
| species reactivity | human |
| application(s) | immunofluorescence: 1-4 µg/mL","immunohistochemistry: 1:50- 1:200 |
| immunogen sequence | GMCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFPVVRGTPKDSCSYLCSHTANRSKFSIADEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSS |
| conjugate | unconjugated |
| UniProt accession no. | Q38SD2, |
| shipped in | wet ice |
| storage temp. | 20°C |
| Gene Information | human ... LRRK1(79705) |
| Personal Protective Equipment | Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 1 |