Spectinomycin

MDL number:	MFCD04041129
NACRES:	NA.85

Кат. номер

S0692-1ML

S0692-1ML-PW

Chemical structure: aminoglycoside
Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.
1 mL in glass bottle
Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.
Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

biological source	Streptomyces sp.
Quality Level	200,
form	solution
storage condition	(Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.)
concentration	100 mg/mL in DMSO/H2O, 1:1
color	colorless
solubility	DMSO/H2O, 1:1: soluble 100 mg/mL
Mode of action	protein synthesis | interferes
antibiotic activity spectrum	Gram-negative bacteria, Gram-positive bacteria, mycobacteria
shipped in	wet ice
storage temp.	20°C

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	201.0 °F - closed cup
Flash Point C	93.9 °C - closed cup