Anti-RKIP Antibody, clone 7F12

eCl@ss:

32160702

Кат. номер

MABC1136-25UG

MABC1136-100UG

Phosphatidylethanolamine-binding protein 1 (UniProt: P30086; also known as PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP) is encoded by the PEBP1 (also known as PBP, PEBP) gene (Gene ID: 5037) in human. RKIP is as member of the phosphatidylethanolamine-binding-protein (PEBP) family that antagonizes multiple cell-survival pathways, such as the Ras-Raf-1, MEK/ERK, NF- B, and G-protein-coupled receptor Kinase 2 and thereby modulates cell growth, apoptosis, motility, invasion and metastasis. Over-expression of RKIP is shown to inhibit c-Src auto-phosphorylation and activation and IL-6-, JAK1 and 2-, and Raf-mediated STAT3 phosphorylation and activation. RKIP overexpression is shown to sensitize tumor cells to chemotherapeutic drug-induced apoptosis. Hence, it is considered as a suppressor of metastasis and a prognostic marker in several cancers. Clone 7F12 specifically detects denatured RKIP endogenously expressed in 293 cells and several pancreatic cell lines. (Ref.: Odabaei, G., et al. (2004). Adv. Cancer Res. 91; 169-200; Yousuf, S., et al. (2014). PLoS One 9(3); e92478).
Specificity
Clone 7F12 specifically detects human Raf kinase inhibitor protein, (RKIP).
Detects Raf kinase inhibitor protein using this mouse monoclonal Anti-RKIP, clone 7F12, Cat. No. MABC1136, tested for use in Western Blotting.
Western Blotting Analysis: A representative lot detected RKIP in Western Blotting applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).
Quality
Evaluated by Western Blotting in HEK293 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected RKIP in HEK293 cell lysates.
Target description
~23 kDa; 21.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Concentration: Please refer to lot specific datasheet.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	7F12, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NP_002558.1,
UniProt accession no.	P30086,
Gene Information	human ... PEBP1(5037)