Anti-Podocin, Mouse monoclonal

NACRES:

NA.41

Кат. номер

SAB4200810-25UL

SAB4200810-100UL

Podocytes are highly specialized visceral epithelial cells that cover the outer aspect of the glomerulus in the kidney. The foot processes of adjacent podocytes regularly interdigitate leaving filtration slits that are bridged by a thin slit diaphragm. This is an extracellular adherens-like junction that serves as a barrier to macromolecules and plays a crucial role in the glomerular filtration process. Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band-7-stomatin family of lipid raft-associated proteins. It is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It interacts there with nephrin (NPHS1 protein), a protein critical in development and function of the kidney filtration barrier and the adapter protein CD2-AP. Podocin is anchored in the glycosphingolipids and cholesterol rich lipid rafts of the outer leaflet of the plasma membrane and is partially localized with the actin cytoskeleton. In vitro studies suggest its involvement in nephrin signaling facilitation via AP-1 in HEK cells. In addition to its presence in the kidney, it was also reported in rat nervous tissue and possibly in vascular smooth muscle.
Synthetic peptide of human Podocin , conjugated to KLH
The antibody may be used in various immunochemical techniques including Immunobloting (~42kDa) and Immunofluorescence. Detection of the Podocin band by Immunoblotting is specifically inhibited by the immunogen.a
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative
This product is for R&D use only, not for drug, household, or other uses.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	PD-65, monoclonal
form	buffered aqueous solution
mol wt	~42 kDa
species reactivity	bovine, human, rat, mouse
packaging	antibody small pack of 25 µL
concentration	1 mg/mL
application(s)	immunoblotting: 0.25-0.5 µg/mL using using human HEK-293T cells.","immunofluorescence: 1-2 µg/mL using human HEK-293T cells.
isotype	IgG2a
UniProt accession no.	Q9NP85,
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable