Anti-Human CD25 (BC96) ColorWheel® Dye-Ready mAb CWA-1021-25UL

Specificity
Clone BC96 is a mouse monoclonal antibody that detects human CD25 (Interleukin-2 receptor subunit a).
Human Interleukin-2 receptor a (CD25).
Anti-Human CD25 (BC96) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Quality Control Testing:
Evaluated by Flow Cytometry in PHA-stimulated human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: Staining of one million 3-day phytohemagglutinin (PHA)-stimulated Human peripheral blood mononuclear cells (PBMC) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1021, Anti-Human CD25 (BC96) Colorwheel® Dye-Ready mAb or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Interleukin-2 receptor subunit alpha (UniProt: P01589; also known as IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25) is encoded by the IL2RA gene (Gene ID: 3559) in human. The IL-2 receptor (IL-2R) is composed of three different subunit complexes, the - (CD25), β- (CD122) and -chains (CD132). By itself, CD25 is not sufficient to trigger the IL-2 signaling pathway because it is a low-affinity form of the IL-2 receptor (KD = 10-8 M) that is non-functional with respect to IL-2 internalization and signaling due to its short cytoplasmic tail. It is mainly involved in IL-2 binding thereby stabilizing a secondary binding site for the β-chain and recruiting -chain to the cell surface. CD25 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-240), a transmembrane domain (aa 241-259), and a cytoplasmic domain (aa 260-272). CD25 is mainly expressed on activated T -cells where it serves as a receptor for Interleukin-2 and is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity that suppresses the activation and expansion of autoreactive T-cells. Mutations in IL2RA gene have been linked to the development of insulin-dependent Diabetes mellitus. (Ref.: Driesen, J., et al. (2008). Immunobiology. 213(9-10); 849-858).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	BC96, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 30.82 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Unknown
Protein ID accession no.	NP_000408.1,
UniProt accession no.	P01589,
shipped in	ambient
storage temp.	2-8°C
Gene Information	mouse ... IL2RA(3559)