EX-CELL® 302 Serum-Free Medium for CHO Cells

MDL number:	MFCD01634638
NACRES:	NA.71

Кат. номер

24326C-10KG

24326C-50K

24326C-100L

24326C-10L

24326C-1L

24326C-500L

24326C-5L

24326C-50KG

24326C-BG

24326C-BIBC

24326C-50L

EX-CELL® 302 is a serum-free medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or GS System™ because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL 302 is to be used with CHO cells transformed using the GS selection system the media needs to be supplemented with GS Supplement 50X for the additional amino acids and nucelosides. For use with the DHFR- selection system we recommend the addition of 4 mM L-Glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.
Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Formulated to contain 21.2 grams of powder per liter of medium
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG is a trademark of Sigma-Aldrich Co. LLC

description	Drug Master File available, for research or for further manufacturing use
Quality Level	500,
sterility	sterile-filtered
form	dry powder
storage condition	protect from light
application(s)	cell culture | mammalian: suitable
pH	7.1-7.7
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3