Taq DNA Polymerase (1 U/l), dNTPack

Enzyme Commission (EC) Number:

2.7.7.7 ( BRENDA | IUBMB )

Кат. номер

4738241001

4738225001

Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.
Taq DNA Polymerase (1 U/µl), dNTPack may be used for:

• PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Packaging
1 kit containing 5 components
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/µl
For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤2,000 reactions (04738241001), sufficient for ≤500 reactions (04738225001)
Quality Level	100,
feature	dNTPs included, hotstart: no
packaging	pkg of 1,000 U (04738241001 [4 x 250 U]), pkg of 250 U (04738225001)
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
optimum pH	~9.0 (20 °C)
shipped in	dry ice
storage temp.	−20°C

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