Laminin 11243217001

CAS Number:	114956-81-9
MDL number:	MFCD00081739

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from , and chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 µg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Quality
Purity: 90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
Sequence
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.
Physical form
Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane
Preparation Note
Working concentration: 2 to 5 µg/cm2
2 to 5 µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
sterility	sterile; sterile-filtered
assay	90% (SDS-PAGE)
form	solution
mol wt	900 kDa
packaging	pkg of 1 mg (2 ml)
Торговая марка	Roche
application(s)	cell culture | mammalian: suitable
UniProt accession no.	Q61292,
shipped in	dry ice
storage temp.	20°C
Gene Information	mouse ... Lamb2(16779)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash