Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)

Кат. номер

10737275001

10127825001

Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.
Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.
Components
EC 2.7.1.1 & 1.1.1.49
Unit Definition
One unit (U) HK will phosphorylate 1 µmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 µmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 µmol of NADH per µmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.
For life science research only. Not for use in diagnostic prodcedures.

Quality Level	100,
form	suspension
packaging	pkg of 10 mL (10737275001 [30 mg]), pkg of 5 mL (10127825001 [15 mg])
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR

NONH for all modes of transport