Expand™ Reverse Transcriptase

Enzyme Commission (EC) Number:

2.7.7.49 ( BRENDA | IUBMB )

Кат. номер

11785834001

11785826001

Expand™ Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.
Specificity
Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Expand™ Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand™ Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.
Features and Benefits

• Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
• No RNase H activity
• Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
• Ideal for two-Step RT-PCR assay
• Purification of cDNA before PCR reaction is not necessary

Packaging
1 set containing 3 components
Unit Definition
One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ x (dT)15 as substrate.
Volume Activity: 50 U/µl
For life science research only. Not for use in diagnostic procedures.
Expand is a trademark of Roche

recombinant	expressed in E. coli (AP401 (k))
Quality Level	100,
form	solution
packaging	pkg of 1,000 U (11785826001 [20 µl]), pkg of 5,000 U (11785834001 [100 µl])
Торговая марка	Roche
parameter	42-43 °C optimum reaction temp.
application(s)	RT-PCR: suitable, RT-qPCR: suitable
input	purified RNA
detection method	probe-based
storage temp.	20°C

RIDADR

NONH for all modes of transport