5-Bromo-2-deoxy-uridine Labeling and Detection Kit II 11299964001

Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Specificity
Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.
The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.
5-Bromo-2-deoxy-uridine Labeling and Detection Kit II has been used in:

• labeling of tooth roots for histology
• immunostaining of mice frontal sections
• immunofluorescence imaging of hepatocellular carcinoma sections

Features and Benefits

• Safe: No radioisotopes are used.
• Easy to perform: Follows a standard immunohistochemistry protocol.
• Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
• Flexible: Allows double-labeling protocols.

Packaging
1 kit containing 7 components.
Principle
Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.
Preparation Note
Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for ≤100 tests
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS02,GHS07,GHS08
signalword	Danger
hcodes	H226 - H312 + H332 - H317 - H319 - H360D
pcodes	P201 - P210 - P261 - P280 - P308 + P313 - P370 + P378
WGK Germany	WGK 2
Flash Point F	136.4 °F
Flash Point C	58 °C