-Gal Staining Set 11828673001

The -Gal Staining Set detects bacterial -Gal activity for direct visualization in transfected cells. Forms a blue precipitate and simplifies the histochemical staining of cells and tissue sections for light microscopy. Upon transfecting cells with a -Gal construct (encoded by the lacZ gene), cells are fixed, washed in PBS (phosphate buffered saline) and stained with freshly prepared staining solution. After staining, cells are washed again in PBS and evaluated by microscopy. When long-term storage is desired, PBS is replaced by mounting media (e.g., glycerol).
For immunohistochemical staining of cells or tissue sections, incubate the specimen with an antibody conjugated to bacterial -galactosidase, according to standard protocols. Subsequently, the specimen is processed for detection of -Gal as described for transfected cells.
Specificity
Bacterial β-galactosidase
The β-Gal Staining Set is used for the histochemical staining of cells and tissue sections demonstrating β-galactosidase activity.
β-Gal staining set has been used in immunofluorescence analysis, senescence assay and histopathologic analysis of tissue samples.
Packaging
1 set containing 2 components.
Specifications
Stability: Stable at +2 to +8°C
For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 100 tests (in 3.5 cm dishes)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash