Pyruvate Kinase (PK)

Enzyme Commission (EC) Number:

2.7.1.40 ( BRENDA | IUBMB )

Кат. номер

10128155001

10128163001

ATP:pyruvate 2-O-phosphotransferase
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.
Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration.
Biochem/physiol Actions
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.
Quality
Contaminants: <0.001% GK, <0.002% “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.)
packaging	pkg of 1 mL (10128155001 [10 mg]), pkg of 10 mL (10128163001 [100 mg])
Торговая марка	Roche
optimum pH	7.0-7.5
shipped in	wet ice
storage temp.	2-8°C

RIDADR

NONH for all modes of transport