Anti-Poly (ADP-Ribose) Polymerase 11835238001

Polyclonal serum (VIC 5) is produced by immunization of rabbits with a full-length recombinant PARP produced in the Sf9/baculovirus system.
IgG class: Rabbit Ig
Number of tests: Variable
Specificity
On Western blots, Anti-PARP recognizes intact PARP from primates or rodents, as well as the large PARP fragment generated by caspases. Anti-PARP will immunoprecipitate intact PARP from primates or rodents.
Poly(ADP-ribose)polymerase (PARP) is a zinc-dependent eukaryotic DNA-binding protein that specifically recognizes DNA-strand breaks that are produced by various genotoxic agents. Recently, it has been reported that PARP serves as a substrate for apoptosis-specific proteases from the ICE family. The protease responsible for the cleavage of PARP was termed apopain, which is derived from its proenzyme Yama/CPP32b.
Additionally, CPP32/Mch2, TX, and Nedd-2 were described as PARP-cleaving ICE-like proteases. It has been found that the 113-kD PARP is cleaved during apoptosis into 89-kD and 24-kD fragments, which could serve as an early specific marker of apoptosis. In immunoprecipitation and western-blot experiments, the antibody recognizes full length or fragmented PARP (primates and rodents).
Species specificity: primates and rodents
Antibody recognizing both the F2 (N-terminal) and catalytic (C-terminal) region (full length polyclonal AB).
Anti-poly (ADP-Ribose) polymerase is used for western blots, immunochemistry, immunofluorescence and immunoprecipitation.
Specifications
Detection of PARP cleavage fragments following apoptosis induction of MCF-7 cells. Extracts from treated and untreated cells were analyzed on western blot, followed by indirect immunodetection with Anti-PARP. Results: Anti- PARP recognizes intact (116 kD) and cleaved (85 kD) PARP.
Lane 1: Untreated control cells.
Lane 2: Cells treated with 100 ng/ml doxorubicin for 24 hours.
Lane 3: Cells treated with 1 mg/ml methotrexate for 24 hours.
Lane 4: Cells treated with 1 mg/ml cytarabin for 24 hours.
Preparation Note
Working concentration: 0.5 µl/ml
0.5 µl/ml (western blot If)
Working solution: Preparation of the solutions

Blocking buffer
50 mM Tris-HCl, pH 8, 150 mM NaCl, 0.3% Tween 20 (v/v), and 5% dry milk.
Immunoreagent
For western blots on nitrocellulose sheets of 8 cm x 8 cm, use 10 µl of serum diluted in 20 ml of blocking buffer. This solution containing the serum can be used five times when stored at -15 to -25 °C.
Extraction buffer for western blots

Per sample, mix:

• 100 µl of 50 mM glucose, 25 mM Tris-HCl, pH 8, 10 mM EDTA, 1 mM PMSF (phenylmethylsulfonyl-fluoride) with
• 50 µl of 50 mM Tris-HCl, pH 6.8, 6 M urea, 6% 2-mercaptoethanol, 3% SDS, 0.003% bromophenol blue.

For life science research only. Not for use in diagnostic procedures.

biological source	rabbit
Quality Level	100,
antibody form	whole antiserum
antibody product type	primary antibodies
clone	polyclonal
form	liquid
packaging	pkg of 100 μL (50 blots)
Торговая марка	Roche
conjugate	unconjugated
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash