Anti-Bromodeoxyuridine-Fluorescein 11202693001

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells undergoing replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, antibromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.
Monoclonal antibody to 5-bromo-2′-deoxyuridine-5′-monophosphate conjugated to fluorescein.
Specificity
The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Antibromo- deoxyuridine does not crossreact with fluorodeoxy- uridine, nor with any endogenous cellular components such as thymidine or uridine.
Apparently, the epitope is inside of the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU.

• Anti-bromodeoxyuridine antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using: Flow cytometry
• Immunohistocytochemistry
• Paraffi sections
• Cryosections

Physical form
Solution, stabilized with phosphate buffered saline, pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin.
Preparation Note
Working concentration: Flow cytometry: 0.5 µg/100 µl (106 cells); Immunohistocytochemistry: 50 µg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Analysis Note
Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine.
No cross reaction to any endogenous thymidine or uridine.
Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	BMC9318, monoclonal
assay	90% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 50 µg (500 µl)
Торговая марка	Roche
isotype	IgG1
conjugate	fluorescein conjugate
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available