Sartorius Vivaspin 20 Starter Pack Polyethersulfone (PES) | 5.000 - 100.00 Centrifugal... VS20S1

Vivaspin 20 Starter Pack Polyethersulfone (PES) | 5.000 - 100.00 Centrifugal unit / pressure driven unit

Membrane: Polyethersulfone (PES)
MWCO: 5.000 - 100.000 Dalton
Type: Centrifugal unit / pressure driven unit
Volume Range: 5 - 20 ml
Certification: None
Vivaspin 20 centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows to monitor the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. "Pressure-fugation" is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.
Swing Bucket Rotor: 20 ml
Fixed Angle Rotor: 14 ml
With Pressure Head: 15 ml
Width: 30 mm
Active Membrane Area: 6.0 cm²
Filtrate vessel: Polycarbonate (PC)
Body: Polycarbonate
Concentrator Cap: Polypropylene (PP)
Pressure Head / Pressure-fuge Head: Acetal / Aluminium
Hold-up volume: <20 µl
Overall length: *With pressure head125 mm*, 116 mm
Membrane material: Polyethersulfone (PES)
Dead stop volume: 50 µl
Rotor Compatibility Please note: Vivaspin 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes
Performance Characteristics

	Time to concentrate up to 30x [min.] at 20°C and solute recovery %
Mode	Centrifuge	Centrifuge	Bench top	Press-fuge
Rotor	Swing bucket	25° Fixed angle	Pressure	Swing bucket
Sart volume	20 ml	14 ml	10 ml	10 ml
	Min./Rec.	Min./Rec.	Min./Rec.	Min./Rec.
Cytochrome c 0.25 mg/ml (12,400 MW)
3,000 MWCO PES	110 / 97 %	180 / 96 %	60 / 96 %	- / -
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES	23 / 99 %	29 / 99 %	50 / 98 %	14 / 98 %
10,000 MWCO PES	16 / 98 %	17 / 98 %	32 / 97 %	8 / 97 %
30,000 MWCO PES	13 / 98 %	15 / 98 %	32 / 97 %	8 / 97 %
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES	27 / 97 %	20 / 95 %	46 / 94 %	13 / 97 %
50,000 MWCO PES	27 / 96 %	22 / 95 %	46 / 93 %	13 / 96 %
100,000 MWCO PES	25 / 91 %	20 / 90 %	42 / 88 %	12 / 94 %
Latex beads 0.004% in DMEM +10% FCS (0.055 μm)
300,000 MWCO PES	20 / 99 %	35 / 99 %	10 / 99 %	- / -
Latex beads 0.004% in DMEM +10% FCS (0.24 μm)
1,000,000 MWCO PES	4 / 99 %	12 / 99 %	4 / 99 %	- / -
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 μm PES	15 / 95 %	5 / 95 %	20 / 95 %	2 / 95 %

Technical data:
Вес нетто:	454 g