- Производитель:
- Sartorius
VIVAFLOW 200 3,000 MWCO PES 3.000 Dalton | Polyethersulfone (PES) 500 - 5.000 ml
MWCO: 3.000 DaltonMembrane: Polyethersulfone (PES)
Volume Range: 500 - 5.000 ml
Vivaflow 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm2. One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaniously.Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single rinse. Vivaflow 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.
Overall length: 126 mm
Overall height: 138 mm
Overall width: 38 mm
Channel width: 10 mm
Channel height: 0,4 mm
Active Membrane Area: 200 cm2
Hold-up volume: 5,3 ml
Minimum Recirculation Volume: < 20 ml
Max. temperature: 60 °C
Non-recoverable Hold-up: < 2 ml
Pump Flow: 200 - 400 ml/min
Operating pressure max.: 4 bar
Main Housing: Acrylic
Flow Channel: Acrylic
Membrane support: Polypropylene (PP)
Seals and O-rings: Silicone
Pressure indicator: Polypropylene, SS spring
Flow Restrictor: Polypropylene (PP)
Fittings: Nylon (NY)
Tubing: PVC (medical grade)
Equipment required The standard Vivaflow 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).
Running 2 Vivaflow 200 cassettes in parallel For speeding up the concentration, two Vivaflow 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows in parallel. High flow rate and recovery Clear housing for process monitoring Ready to use single cassettes Easy set up of 2 cassettes Re-usable product
Performance Characteristics
Time to concentrate up to 20x (min.) at 3 bar inlet pressure, 20°C | ||||
---|---|---|---|---|
Start volume | Average flux | Recovery % | ||
1 litre | ml/min | Direct | 25 ml rinse | |
Lysozyme 0.25 mg/ml (14,000 MW) | ||||
2,000 MWCO Hydrosart | 160 | 6 | 97 % | > 99 % |
3,000 MWCO PES | 180 | 5 | 97 % | > 99 % |
BSA 1.0 mg/ml (66,000 MW) | ||||
5,000 MWCO PES | 29 | 33 | 98 % | > 99 % |
5,000 MWCO Hydrosart | 70 | 14 | 98 % | > 99 % |
10,000 MWCO PES | 23 | 41 | 96 % | > 99 % |
10,000 MWCO Hydrosart | 35 | 27 | 98 % | > 99 % |
30,000 MWCO PES | 25 | 38 | 96 % | 99 % |
30,000 MWCO Hydrosart | 20 | 48 | 96 % | > 99 % |
50,000 MWCO PES | 22 | 43 | 96 % | 98 % |
γ Globulins 1.0 mg/ml (average 160,000 MW) | ||||
100,000 MWCO PES | 54 | 18 | 96 % | 99 % |
100,000 MWCO RC | 45 | 21 | 96 % | 99 % |
Yeast 1.0 mg/ml (S. Cerevisiae) | ||||
0.2 μm PES | 11 | 86 | 92 % | 98 % |
Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES | ||||
BSA 0.001 mg/ml | 18 | 52 | 90 % | 98 % |
BSA 0.01 mg/ml | 20 | 47 | 92 % | 98 % |
BSA 0.1 mg/ml | 21 | 45 | 94 % | 99 % |
Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES | ||||
BSA 1.0 mg/ml (66,000 MW) | 67 | 70 | 97 % | > 99 % |
Technical data: | |
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Вес нетто: | 1,159 kg |
Страна происхождения: | United kingdom |
Вес брутто: | 1,159 kg |
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