SAFC

Quality Level	100
vapor density	1.03 (vs air)
vapor pressure	52 mmHg ( 37 °C)
form	liquid
autoignition temp.	572 °F
contains	10-15% methanol as stabilizer
concentration	37 wt. % in H₂O, 37-38%
application(s)	tissue processing: suitable
impurities	0.03% free acid (as HCOOH)
color	APHA: 10
pH	2.8-4.0
bp	97 °C
mp	-15 °C
density	1.09 g/mL at 25 °C (lit.)
SMILES string	[H]C([H])=O
InChI	1S/CH2O/c1-2/h1H2
InChI key	WSFSSNUMVMOOMR-UHFFFAOYSA-N

Finding the right excipient that matches your needs as well as regulatory demands can be a complicated challenge in formulation. With our application know-how and regulatory expertise, we support you in every step of development, scale-up, and production.
As part of our Emprove® Program, our raw materials are offered with extensive documentation facilitating compliance of your pharma and biopharma product, full supply chain transparency and risk mitigation. Our SAFC® portfolio of high-quality products for biopharmaceutical and pharmaceutical formulation and production withstands strict quality control procedures and is produced according to applicable cGMP guidelines.

pictograms	GHS02,GHS05,GHS06,GHS08
signalword	Danger
hcodes	H226 - H301 + H311 - H314 - H317 - H330 - H335 - H341 - H350 - H370
pcodes	P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Target Organs	Respiratory system
RIDADR	UN 2209 8 / PGIII
WGK Germany	WGK 3
Flash Point F	133.0 °F - closed cup
Flash Point C	56.11 °C - closed cup

Узнать цену

Подробнее

Ethyl acetate

Empirical Formula (Hill Notation):	C₄H₈O₂
Linear Formula:	CH₃COOC₂H₅
CAS Number:	141-78-6
Molecular Weight:	88.11
Beilstein/REAXYS Number:	506104
MDL number:	MFCD00009171
EC Index Number:	205-500-4

Кат. номер
1008649181
1008641000
1008640000
1008646190
1008642500BWT
1008646010
1008642500
1008649950
1008649999
1008646025
1.00864.9181
1.00864.6190
1.00864.6025
1.00864.6010
1.00864.2500.BWT
1.00864.2500
1.00864.1000
MX11008642500
CO1E1008641000

General description

Application

Ethyl acetate is used as solvent in topical, and oral for formulations and can also be used as a flavoring agent.

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Supplement with 0.292 g/L L-glutamine.

storage conditions	Store at +15°C to +25°C.
Quality Level	500
Agency/Method	BP, NF, Ph. Eur.
vapor density	3 (20 °C, vs air)
vapor pressure	73 mmHg ( 20 °C)
product line	EMPROVE^® ESSENTIAL
assay	98.0-102.0% (GC)
form	liquid
autoignition temp.	801 °F
potency	5620 mg/kg LD₅₀, oral (Rat), >18000 mg/kg LD₅₀, skin (Rabbit)
expl. lim.	2.2-11.5 %, 38 °F
refractive index	n20/D 1.3720 (lit.)
bp	76.5-77.5 °C (lit.)
mp	84 °C (lit.)
transition temp	flash point -4 °C
solubility	85.3 g/L
density	0.902 g/mL at 25 °C (lit.)
storage temp.	2-30°C
SMILES string	CCOC(C)=O
InChI	1S/C4H8O2/c1-3-6-4(2)5/h3H2,1-2H3
InChI key	XEKOWRVHYACXOJ-UHFFFAOYSA-N

Узнать цену

Подробнее

Basal Medium Eagle

Quality Level	400,
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	L-glutamine: no Earle’s salts (5% CO₂): yes NaHCO₃: yes
shipped in	ambient
storage temp.	2-8°C

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1

Узнать цену

Подробнее

DMEM/Nutrient Mixture F-12 Ham

MDL number:	MFCD00217417
NACRES:	NA.75

Formulated to contain 14.8 grams of powder per liter of medium.

Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

form	powder
application(s)	cell culture \| mammalian: suitable
components	phenol red: no NaHCO₃: no sodium pyruvate: 0.055 g/L glucose: 3.15 g/L HEPES: 15 mM L-glutamine: no
shipped in	ambient
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

Dulbeccos Modified Eagles Medium/Hams Nutrient Mixture F12

MDL number:	MFCD00217417
NACRES:	NA.71

description	for research or for further manufacturing use
Quality Level	500,
form	powder
components	phenol red: yes glucose: 3.15 g/L sodium pyruvate: 0.055 g/L L-glutamine: yes
shipped in	ambient
storage temp.	2-8°C

Dulbeccos Modified Eagles Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

Dulbeccos Modified Eagles Medium/High Modified

Formulated to contain 13.5 grams of powder per liter of medium.

description	for research or for further manufacturing use
Quality Level	500,
form	powder
application(s)	cell culture \| mammalian: suitable
components	sodium pyruvate: yes L-glutamine: yes phenol red: yes glucose: high
shipped in	ambient
storage temp.	2-8°C

EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® 293 Serum-Free Medium for HEK 293 Cells

MDL number:	MFCD01634638
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile
form	liquid
storage condition	protect from light
application(s)	cell culture \| mammalian: suitable
components	glucose: 6.0 g/L NaHCO₃: 1.8 g/L HEPES: no L-glutamine: no
shipped in	ambient
storage temp.	2-8°C

EX-CELL® 302 is a serum-free medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or GS System™ because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL 302 is to be used with CHO cells transformed using the GS selection system the media needs to be supplemented with GS Supplement 50X for the additional amino acids and nucelosides. For use with the DHFR- selection system we recommend the addition of 4 mM L-Glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® 302 Serum-Free Medium for CHO Cells

MDL number:	MFCD01634638
NACRES:	NA.71

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Formulated to contain 21.2 grams of powder per liter of medium

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG is a trademark of Sigma-Aldrich Co. LLC

description	Drug Master File available, for research or for further manufacturing use
Quality Level	500,
sterility	sterile-filtered
form	dry powder
storage condition	protect from light
application(s)	cell culture \| mammalian: suitable
pH	7.1-7.7
storage temp.	2-8°C

EX-CELL™ 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media (Catalog No. 14324C and 14326C) can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System™, and DHFR- selection systems).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Узнать цену

Подробнее

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells

MDL number:	MFCD01634638
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use, without L-glutamine
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
components	phenol red: yes glucose: 3.45 g/L L-glutamine: no HEPES: 7.5 mM NaHCO₃: 1.6 g/L
shipped in	ambient
storage temp.	2-8°C

Baculoviruses are powerful expression systems for production of recombinant proteins. The most well known and highly investigated baculovirus vector expression system (BEVS) uses Spodoptera frugiperda cells (especially Sf9) as the host cell of choice for infection with recombinant Autographa californica nuclear polyhedrosis virus for heterologous protein expression. Recent investigations have shown that other cell lines may be better expression substrates than spodopteran cells. The BTI-TN-5B1-4 clone of Trichoplusia ni, commonly referred to as High Five™, is reported to be a superior host substrate for the expression of selected recombinant proteins using BEVS. This cell line portrays strong adherent characteristics, but can be adapted to suspension growth.
EX-CELL™ 405 is a serum-free medium for optimal growth and viability of BTI-TN-5B1-4 (Tn5, High Five) cells. In addition, EX-CELL 405 supports recombinant protein production in High Five cells to higher levels than reported for Sf9 cells. EX-CELL 405 is a complete medium. No protein supplements need to be added to this medium prior to use.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® 405 Serum-Free Medium for Insect Cells

MDL number:	MFCD01634638
NACRES:	NA.75

Available in USA, Canada, and Europe. Please check with your local supplier regarding availability and restrictions in other countries.
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

description	for research or for further manufacturing use
Quality Level	500,
sterility	sterile-filtered
form	liquid
quality	Drug Master File available
application(s)	cell culture \| insect: suitable
components	HEPES: no L-glutamine: yes: 4.717 g/L glucose: 9.434 g/L NaHCO₃: 0.33 g/L
shipped in	ambient
storage temp.	2-8°C

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 x 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® 420 Serum-Free Medium for Insect Cells

MDL number:	MFCD01634638
NACRES:	NA.75

Available in USA, Canada, and Europe. Please check with your local supplier regarding availability and restrictions in other countries.
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

description	for research or for further manufacturing use
Quality Level	500,
sterility	sterile-filtered
form	liquid
quality	Drug Master File available
application(s)	cell culture \| insect: suitable
components	glucose: 6.0 g/L NaHCO₃: 0.35 g/L HEPES: no L-glutamine: 1.0 g/L
shipped in	ambient
storage temp.	2-8°C

EX-CELL 610-HSF is a low-protein (11 mg/L) serum-free medium. Originally developed to support the growth of hybrid cells in culture, it has been shown to support a wide range of cells including lymphoid and epithelial cells and B cell hybridomas of murine, rat and human origin. EX-CELL 610-HSF has been used in stationary culture systems and in large-scale bioreactors. In both types of culture, the production of cellular products, particularly monoclonal antibodies, has been shown to equal or exceed levels seen when the same cells are cultured in the presence of Fetal Bovine Serum (FBS).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® 610-HSF Serum-Free Medium for Hybridoma Cells

MDL number:	MFCD03098239
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	Drug Master File available, for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
storage condition	protect from light
application(s)	cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
components	NaHCO₃: 1.60 g/L HEPES: 7.5 mM glucose: 3.45 g/L L-glutamine: 0.333 g/L
shipped in	ambient
storage temp.	2-8°C

EX-CELL 620-HSF is a low-protein (approximately 11 mg/L) serum-free medium which has been specifically developed for the long-term growth of hybridoma and related cells capable of expressing monoclonal antibodies and other protein products. The following hybridoma, lymphoma and myeloma cell lines have demonstrated sustained long-term, serum-free growth (> 15 passages) in EX-CELL 620-HSF: Sp2/0, Ag14, L243, VD-10, Hut 78, K562. Additionally, transformed Chinese Hamster Ovary (CHO) cells have demonstrated excellent growth and productivity when grown in this medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® 620-HSF Serum-Free Medium for Hybridoma Cells

MDL number:	MFCD01634638
NACRES:	NA.75

Available in USA, Canada, and Europe. Please check with your local supplier regarding availability and restrictions in other countries.
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
components	L-glutamine: no NaHCO₃: 1.6 g/L HEPES: 7.5 mM glucose: 3.45 g/L
shipped in	ambient
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® ACF CHO Medium

MDL number:	MFCD01634638
NACRES:	NA.75

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity.

Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Powdered media are extremely hygroscopic and should be protected from atmospheric moisture. Preparing a concentrated solution of medium is not recommended as precipitates may form.
1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC.
2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC.
3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2.
4. Stir until dissolved.
5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine.
6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L).
7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH.
8. Use additional water to bring the solution to final volume.
9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane.
10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

description	for research or for further manufacturing use
form	dry powder
quality	Drug/Device Master File available
concentration	21.5 g/L
application(s)	cell culture \| mammalian: suitable
storage temp.	2-8°C

EX-CELL® AdvancedCHO Fed-batch Medium is chemically-defined and contains no animal derived components. This newest platform from SAFC has been specifically developed to support growth and productivity across a diverse set of Chinese Hamster Ovary (CHO) cells in fed-batch cultures while still allowing for flexibility in the adjustment of protein quality attributes. This contemporary fed-batch platform was developed using a strategy of Multivariate Analysis to establish correlations in which the formulation components influence critical process and product attributes such as titer, fold expansion, receptor expression, and glycosylation. This platform contains no hypoxanthine and thymidine, L-glutamine, phenol red or 2-mercaptoethanol (2-ME).
This medium is intended to be a part of a fed-batch platform, and used with EX-CELL® Advanced CHO Feed 1 (24367C - with glucose or 24368C - without glucose).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® Advanced CHO Fed-batch Medium

Packaging

100 L in poly drum
24366C-1L
24366C-10L

Linkage

Submit the EX-CELL Advanced CHO Fed-batch request form to get in touch with a SAFC representative.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500
description	Animal-component free, Chemically defined
form	powder
application(s)	cell culture \| mammalian: suitable (suspension)
impurities	endotoxin, tested
shipped in	ambient
storage temp.	2-8°C

EX-CELL® Advanced HD Perfusion Medium is chemically-defined and contains no animal derived components. The new medium has been specifically developed to support growth and productivity across a diverse set of Chinese Hamster Ovary (CHO) cells in perfusion cultures while still allowing for flexibility in the adjustment of protein quality attributes. This medium was developed using a strategy of Multivariate Analysis to establish correlations in which the formulation components influence critical process and product attributes such as titer, fold expansion, protein expression and glycosylation.

This medium is intended to be used in perfusion processes. While the medium was developed specifically for steady-state perfusion, it can also support other perfusion applications such as N-1, continuous fed-batch and production perfusion where cells are not bled.

Pictograms	GHS07
Signal Word	Warning
Hazard Statements	H319
Precautionary Statements	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Узнать цену

Подробнее

EX-CELL® Advanced HD Perfusion Medium

Packaging

24370C-5L Bottle
24370C-200L PE-Drum
24370C-500L PE- Drum
24370C-800L Dry Powder bag

Additional sizes upon request

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500,
description	Animal-component free, Chemically defined
form	powder
application(s)	cell culture \| mammalian: suitable (suspension)
impurities	endotoxin, tested
shipped in	ambient
storage temp.	2-8°C

EX-CELL ® CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells.

This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up, and to provide an appropriate medium for the culture of CHO cells using the Glutamine Synthetase, or GS System™. This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase (DHFR) gene amplification systems.

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Узнать цену

Подробнее

EX-CELL® CD CHO Fusion

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
components	L-glutamine: no
shipped in	ambient
storage temp.	2-8°C

EX-CELL® CD CHO is a chemically defined, animal-component free, serum-free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells and expression of antibodies or protein products in suspension cell culture. CHO suspension cultures can be subcultured directly into EX-CELL CD CHO from serum-supplemented or serum-free medium with little or no adaptation. EX-CELL CD CHO is formulated without hypoxanthine, thymidine or L-glutamine, making it an appropriate medium for selection systems such as DHFR- and Glutamine Synthetase (GS System). For applications that do not require the selective pressure of a hypoxanthine/thymidine (HT)-deficient medium, we recommend the use of EX-CELL CD CHO with hypoxanthine and thymidine.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Узнать цену

Подробнее

EX-CELL® CD CHO Serum-Free Medium for CHO Cells

MDL number:	MFCD01634638
NACRES:	NA.71

Formulated to contain 19.3 grams per liter of medium.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500,
form	dry powder
application(s)	cell culture \| mammalian: suitable
storage temp.	2-8°C

EX-CELL CD CHO is a chemically defined, animal-component free, serum-free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells and expression of antibodies or protein products in suspension cell culture. CHO suspension cultures can be subcultured directly into EX-CELL CD CHO from serum-supplemented or serum-free medium with little or no adaptation. EX-CELL CD CHO (Catalog No. 14361C) is formulated with hypoxanthine and thymidine, making it an appropriate medium for applications that do not require the selective pressure of a hypoxanthine/thymidine (HT)-deficient medium, such as CHO-K1 and Glutamine Synthetase (GS System™) users.

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

MDL number:	MFCD01634638
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
GS System is a trademark of Lonza Group Ltd.

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
components	glucose: 5.6 g/L phenol red: no HEPES: no NaHCO₃: 2.10 g/L
shipped in	ambient
storage temp.	2-8°C

Complete, chemically-defined medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® CD CHO-3 Medium, Chemically Defined

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. This medium supports and maintains high cell densities for extended periods, resulting in increased productivity.

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, transferrin, hydrolysates, or other undefined nutrients or supplements. Contains no animal-derived components or components synthesized from animal-derived materials.

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Aseptically add 20-40 mL of 200 mM L-glutamine solution per liter of medium prior to use.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	HEPES: yes L-glutamine: no
shipped in	ambient
storage temp.	2-8°C

This animal-component free formulation is optimized for high protein production and well as superior expansion of CHO cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® CHO 5 Medium

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
suitability	endotoxin tested
components	NaHCO₃: yes L-glutamine: yes HEPES: yes
shipped in	ambient
storage temp.	2-8°C

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® CHO Cloning Medium

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Proprietary formulation containing inorganic salts, sodium bicarbonate, essential and non-essential amino acids, vitamins, trace elements, plant hydrolysates, and other organic compounds.
Does not contain antibiotics, antimycotics, L-glutamine, insulin, or transferrin.

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
sterility	sterile-filtered
form	liquid
storage condition	protect from light
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	L-glutamine: no NaHCO₃: yes
shipped in	ambient
storage temp.	2-8°C

This animal component-free medium (containing no hypoxanthine or thymidine) is formulated to maximize cell growth and recombinant protein production using the Dihydrofolate Reductase (DHFR) gene amplification system in DHFR- Chinese Hamster Ovary (CHO) cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® CHO DHFR Medium

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. This medium supports and maintains high cell densities for extended periods, resulting in increased productivity.

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	NaHCO₃: yes phenol red: no HEPES: yes glucose: 5.4 g/L L-glutamine: no
shipped in	ambient
storage temp.	2-8°C

EBx is a trademark of Vivalis SA
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® CHOZN® Platform Medium

MDL number: MFCD01634638

NACRES: NA.71

Кат. номер

24330C-ALC

24330C-ALC2

24330C-25KG

24330C-25K

24330C-BG

24330C-10L

24330C-BIBC

24330C-100L

24330C-1L

24330CLF1-BIBC

Application
EX-CELL CHOZN Platform Medium is a chemically defined, protein and animal component free medium. This medium, in conjunction with the EX-CELL
CHOZN Platform Feed, was developed to enhance the production of recombinant Chinese Hamster Ovary (CHO) cells engineered to produce recombinant
proteins in suspension cultures. This medium has been optimized for r-protein producing clones that were developed using SAFCs CHOZN GS cell line.
Legal Information
CHOZN is a registered trademark of Sigma-Aldrich Co. LLC
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Параметры

Quality Level 500,

description animal-component free, without L-glutamine

form dry powder

quality cGMP

application(s) cell culture | mammalian: suitable

storage temp. 2-8°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany WGK 2

Flash Point F Not applicable

Flash Point C Not applicable

Узнать цену Получить предложение
Подробнее

EX-CELL® EBx PRO-II Serum-Free Media

Quality Level	400,
description	Animal-component-free, low protein, for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
availability	not available in Japan
application(s)	cell culture \| mammalian: suitable
components	phenol red: no L-glutamine: no
shipped in	wet ice
storage temp.	2-8°C, 2-8°C

EX-CELL® EBx™ GRO-I Serum-Free Media has been used to culture EB66 cell line derived from duck embryonic stem cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® EBx™ GRO-I Serum-Free Media

EBx is a trademark of Vivalis SA
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	Animal-component-free, low protein, for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
availability	not available in Japan
application(s)	cell culture \| mammalian: suitable, cell culture \| stem cell: suitable
components	L-glutamine: no
shipped in	ambient
storage temp.	2-8°C, 2-8°C

Formulated to contain 19.1 grams of powder per liter of medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® EBx™ GRO-I Serum-Free Medium for Embryonic Stem Cells

EBx is a trademark of Vivalis SA
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500,
description	Animal-component free, Animal-component-free, low protein, for research or for further manufacturing use
form	dry powder
application(s)	cell culture \| mammalian: suitable
storage temp.	2-8°C, 2-8°C

EBx is a trademark of Vivalis SA
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® EBx™ PRO-I Serum-Free Media

Quality Level	400,
description	Animal-component-free, low protein, for research or for further manufacturing use
form	liquid
availability	not available in Japan
application(s)	cell culture \| mammalian: suitable
components	phenol red: no L-glutamine: no
shipped in	wet ice
storage temp.	2-8°C, 2-8°C

Quantity
Formulated to contain 20.7 grams of powder per liter of medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® EBx™ PRO-I Serum-Free Medium for Embryonic Stem Cells

EBx is a trademark of Vivalis SA
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

description	Animal-component-free, low protein, for research or for further manufacturing use
Quality Level	500,
form	dry powder
application(s)	cell culture \| mammalian: suitable
storage temp.	2-8°C, 2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® GTM-3

Complete, ready-to-use medium that requires only the addition of L-glutamine for the growth of HEK-293 cells, retinoblastoma-like cells and propagation of adenoviruses. Additionally, the medium is designed to support growth of cells in suspension culture with minimal levels of cell clumping. Developed to meet the needs of biotechnology and vaccine manufacturing.

Do not freeze.

Prior to use medium should be warmed to 37°C and supplemented with 20ml/L of 200mM L-glutamine.

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	glucose: 2.93 g/L
shipped in	ambient
storage temp.	2-8°C

EX-CELL HeLa is an animal-protein free, serum-free liquid medium developed for the long-term growth of HeLa cells in suspension culture. The HeLa cell line is a clonal derivative of the parent HeLa cell line and is capable of growth in suspension culture. HeLa suspension cultures can be subcultured directly into EX-CELL HeLa from serum-supplemented or serum-free media. Suspension cultures in EX-CELL HeLa have been carried for more than 25 passages with no loss of growth or viability.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® HeLa Serum-Free Medium for HeLa Cells

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use, serum-free
sterility	sterile
form	liquid
application(s)	cell culture \| mammalian: suitable
components	HEPES: no L-glutamine: no glucose: 6.0 g/L NaHCO₃: 1.8 g/L
shipped in	ambient
storage temp.	2-8°C

EX-CELL MDCK is an animal-protein free, serum-free medium developed for the long-term growth of Madin Darby Canine Kidney (MDCK) and related cells. The cells, in an attachment culture, can be subcultured directly into EX-CELL MDCK from serum-supplemented media without adaptation. Cell densities and doubling times achieved under serum-free conditions are comparable to those achieved in a serum-supplemented culture.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® MDCK Serum-Free Medium for MDCK Cells

MDL number:	MFCD01635225
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	500,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
components	NaHCO₃: 1.8 g/L HEPES: no glucose: 6.0 g/L L-glutamine: no
shipped in	ambient
storage temp.	2-8°C

EX-CELL NS0 is an animal-component free, protein-free, chemically defined, serum-free medium developed for the long-term growth of NS0-related cells in suspension culture. The NS0 cells are clonal derivatives of the parent NS1 cell line and are capable of growth in suspension culture. NS0 hybridoma suspension cultures can be subcultured direclty into EX-CELL NS0 from serum-supplemented or serum-free media. Suspension cultures in EX-CELL NS0 have been carried for more that 50 passages with no loss of growth or viability.

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® NS0 Serum-Free Medium for NS0 Cells

MDL number:	MFCD08705554
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
components	glucose: 5.6 g/L L-glutamine: no HEPES: no NaHCO₃: 2.14 g/L
shipped in	ambient
storage temp.	2-8°C

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Узнать цену

Подробнее

EX-CELL® Sp2/0 Serum-Free Medium for Sp2/0 Cells

MDL number:	MFCD08705555
NACRES:	NA.75

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
components	L-glutamine: no NaHCO₃: 1.05 g/L glucose: 4.0 g/L HEPES: 7.5 mM
shipped in	ambient
storage temp.	2-8°C

EX-CELL VPRO is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Retinoblast cells (PER.C6™ and related cell lines). The cells can be subcultured directly into EX-CELL VPRO from serum-free media with little or no adaptation. PER.C6 cells can be grown as suspension cultures either in shaker flasks or roller bottles, with roller bottles being the preferred culture system. Suspension cultures can be maintained, without refeeding, for approximately 10 days and can be carried for more than 20 passages with no loss of viability.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

EX-CELL® VPRO Serum-Free Medium for Retinoblast Cells

MDL number:	MFCD08705556
NACRES:	NA.75

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
description	for research or for further manufacturing use
sterility	sterile-filtered
form	liquid
storage condition	protect from light
application(s)	cell culture \| mammalian: suitable
components	NaHCO₃: 1.8 g/L L-glutamine: no HEPES: no glucose: 6.0 g/L
shipped in	ambient
storage temp.	2-8°C

Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

Hams Nutrient Mixture F12

MDL number:	MFCD00217417
NACRES:	NA.75

description	for research or for further manufacturing use, with 1.0 mM L-glutamine
Quality Level	500,
sterility	sterile
form	liquid
quality	for further manufacturing use
application(s)	cell culture \| mammalian: suitable
components	phenol red: 1.274 mg/L NaHCO₃: 1176 mg/L sodium pyruvate: 110.1 mg/L L-glutamine: 146.2 mg/L
shipped in	ambient
storage temp.	2-8°C

Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

Iscoves Modified Dulbeccos Medium I6529-500ML

MDL number:	MFCD00217469
NACRES:	NA.75

Application

Quality Level	400,
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	glucose: yes phenol red: yes NaHCO₃: yes L-glutamine: yes
shipped in	ambient
storage temp.	2-8°C

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

Medium 199 Modified

Components

Contains all L-form amino acids

Formulated to contain 9.5 grams of powder per liter of medium.

description	for research or for further manufacturing use
Quality Level	500,
form	dry powder
application(s)	cell culture \| mammalian: suitable
components	Earle’s salts (5% CO₂): yes phenol red: 21.42 mg/L L-glutamine: 100 mg/L NaHCO₃: no
shipped in	ambient
storage temp.	2-8°C

Reduces FBS or other serum usage from 10% to 3%.

Personal Protective Equipment	Eyeshields,, Faceshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

MegaCell™ Dulbeccos Modified Eagles Medium

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.

Quality Level	400,
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	glucose: 4500 mg/L (Dextro) L-glutamine: no sodium pyruvate: yes
shipped in	ambient
storage temp.	2-8°C

Formulated to contain 9.6 grams of powder per liter of medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

MegaCell™ RPMI-1640 Medium
NACRES: NA.75

Кат. номер

M3817-PH

M3817-500ML

General description
MegaCell™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.
Application
MegaCell™ RPMI-1640 Medium has been used:
as a component of the TSC medium for culturing trophoblast stem cells (TSC)
as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

Reconstitution
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
Legal Information
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
Параметры

Quality Level 500,

sterility sterile-filtered

form liquid

application(s) cell culture | mammalian: suitable

impurities endotoxin, tested

components L-glutamine: no

shipped in ambient

storage temp. 2-8°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany WGK 3

Flash Point F Not applicable

Flash Point C Not applicable
Узнать цену Получить предложение
Подробнее

Minimum Essential Medium

Quality Level	400,, 500,
description	for research or for further manufacturing use
form	dry powder
application(s)	cell culture \| mammalian: suitable
components	Earle’s salts (5% CO₂): yes L-glutamine: 292 mg/L NaHCO₃: no phenol red: 10.620 mg/L
shipped in	ambient
storage temp.	2-8°C

Formulated to contain 11.0 grams of powder per liter of medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

Minimum Essential Medium Jokliks Modified

description	for research or for further manufacturing use
Quality Level	500,
form	dry powder
application(s)	cell culture \| mammalian: suitable
components	phenol red: 10.620 mg/L NaHCO₃: no Earle’s salts (5% CO₂): yes L-glutamine: 292 mg/L
shipped in	ambient
storage temp.	2-8°C

NCTC-109 and the 135 modification were formulated to establish and maintain a strain of mouse cells (L929) in a chemically defined and serum free medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Узнать цену

Подробнее

NCTC 109 Medium

Supplement with 0.14 g/L L-glutamine.

Quality Level	400,, 500,
sterility	sterile-filtered
form	liquid
application(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
components	L-glutamine: no NaHCO₃: 2.2 g/L phenol red: 0.02 g/L glucose: 1.0 g/L
shipped in	ambient
storage temp.	2-8°C