GenElute™ Endotoxin-free Plasmid Midiprep Kit PLED35-1KT

NACRES:

NA.52

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with 0.1 EU/µg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 µg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
The kit has been used for purifying plasmid DNA, which has been used for transfection.

• High transfection efficiency and yield
• Purify up to 250 µg endotoxin-free plasmid DNA (0.1 EU/µg DNA)
• Fast and simple allowing up to 12 preps in less than 2 hours

GenElute is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200
usage	sufficient for 35 purifications
greener alternative product characteristics	Inherently Safer Chemistry for Accident Prevention Learn more about the Principles of Green Chemistry.
storage temp.	15-25°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H290 - H302 + H332 - H315 - H319 - H334 - H336
pcodes	P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Target Organs	Central nervous system
RIDADR	UN 3316 9
Flash Point F	Not applicable
Flash Point C	Not applicable