Ribonuclease A from bovine pancreas

CAS Number:	9001-99-4
Enzyme Commission (EC) Number:	3.1.27.5 ( BRENDA | IUBMB )
EC Number:	232-646-6
MDL number:	MFCD00132181
NACRES:	NA.54

Кат. номер

R5503-1G-C

R5503-500MG-C

R5503-5G-C

R5503-10G-C

R5503-5G

R5503-10G

R5503-1G

R5503-500MG

R5503-100MG

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

• RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
• RNase A is also used in RNA sequence analysis and protection assays.
• RNase A has been used as a tool for computer-aided drug design.
• RNase A supports the analysis of RNA sequences.
• RNase A hydrolyze RNA contained in protein samples.
• Purification of DNA is supported by RNase A.

100, 500 mg in poly bottle
1, 5, 10 g in poly bottle
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3 phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
Salt fractionated and chromatographically purified.
Protein determined by E.

Quality Level	200
type	Type I-AS
form	lyophilized powder
specific activity	50-100 Kunitz units/mg protein
mol wt	~13,700
impurities	salt, essentially free
Featured Industry	Diagnostic Assay Manufacturing
foreign activity	protease, essentially free
storage temp.	−20°C

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P501
Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable