GenomePlex® Complete Whole Genome Amplification (WGA) Kit

NACRES:

NA.55

Кат. номер

WGA2-10RXN

WGA2-50RXN

WGA2-500RXN

GenomePlex Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
GenomePlex® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including:

• microarray analysis
• SNP analysis
• STR analysis
• DNA archiving

• Starting material: 10 ng gDNA or damaged DNA or 1 µl blood or cheek swab
• Expected yield: 5 - 10 µg
• Time required: ~3 hours (14 cycles in thermocycler)
• WGA DNA polymerase offers increased amplification accuracy resulting in more accurate downstream applications
• Complete representation of the entire genome with minimal allele bias
• No amplicon in the negative control reactions
• DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)

The sequences of the universal primers provided in this kit are considered proprietary.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchasers own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchasers activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Quality Level	200
application(s)	whole genome amplification: suitable
shipped in	wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3