MDL number: | MFCD00165844 |
NACRES: | NA.25 |
Кат. номер |
S8445-10VL-PW |
S8445-1VL-PW |
S8445-PH |
S8445-1VL |
S8445-10VL |
SigmaMarker has been used as a protein marker in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Will yield 12 protein bands with Coomassie Blue staining.
Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
-Galactosidase,
E. coli (116,000)
Myosin, porcine heart (200,000)
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
Quality Level | 200 |
form | lyophilized powder |
usage | sufficient for 10-20 large gels (16 x 18 cm), sufficient for 20-30 mini-gels (10 x 10 cm), 1 vial sufficient for 50-100 PhastGels |
mol wt | 6,500-200,000 Da |
storage temp. | 2-8°C |
pictograms | GHS08 |
signalword | Danger |
hcodes | H315-H319-H334 |
pcodes | P261-P284-P304 + P340-P305 + P351 + P338-P342 + P311 |
Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |