Live Cell Imaging Reagents
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ATP Cell Viability Luciferase Assay SCT149
General descriptionBecause ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP available. The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. This kit takes advantage of Firefly luciferase’s use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available in cell cultures. The sensitive assay procedure involves a single addition of ATP detection cocktail directly to cells cultured in a serum-supplemented medium. No cell washing, medium removal and multiple pipetting are required. The kit can be used to detect as little as a single cell or 0.01 picomoles of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.
The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. The luminescence signal generated is stable for about 1 minute.ApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Signaling
Cell Viability Assay
A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability.Components1) D-Luciferin (CS224519): 4 X 10 mg
2) Firefly Luciferase (CS224520): 5 X 200 µ
3) ATP Assay Buffer (CS224521): 1 X 10 mL
4) ATP, 2µM (CS224522): 1 X 100 µLPhysical formLiquidStorage and StabilityStore ATP Cell Viability Luciferase Assay at -80°C. Avoid repeated freeze-thaw cycles. Product is stable for at least 6 months from date of receipt when stored as recommended.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method chemiluminescent shipped in ambient Узнать цену -
BioTracker 400 Blue Cytoplasmic Membrane Dye SCT109
General descriptionLipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 366nm
Emission: 441nmApplicationResearch Sub Category
Live Cell Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Live cell fluorescent imagingComponents1. 1 vial of 250µL Cell Labeling Solution (CS224586)
2. 1 vial of 250µL Loading Buffer (CS224587)QualitySpectral Properties
Absorbance: 366nm
Emission: 441nmPhysical formLiquidStorage and StabilityStore BioTracker 400 Blue Cytoplasmic Membrane Dye at room temp. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric Узнать цену -
BioTracker 405 Blue Mitochondria Dye SCT135
General descriptionMitochondria are organelles found in large numbers in most cells, in which the biochemical processes of respiration and energy production occurs. In metabolically active cells, mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.
BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is lost when mitochondria become depolarized during cell death, allowing monitoring of cell viability.
The BioTracker 405 Blue Mitochondria Dye can be detected using the blue DAPI channel.
Spectral Properties
Absorbance: 398nm
Emission: 440nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health.QualitySpectral Properties
Absorbance: 398nm
Emission: 440nmPhysical formLyophilizedStorage and StabilityStore BioTracker 405 Blue Mitochondria Dye at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 488 Green CSFE Cell Proliferation Kit SCT110
General descriptionBioTracker SE Cell Proliferation Kits diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining. Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader.
Spectral Properties
Absorbance: 495nm
Emission: 519nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging cell proliferation kit based on carboxyfluorescein succinimidyl ester (CFSE) used for fluorescent cell labeling in flow cytometry applications.Components1. 10 vials of BioTracker 488 Green CFSE Dye (50µg) (CS224588)
2. 1 vials of Anhydrous DMSO (500µL) (CS224578)QualitySpectral Properties
Absorbance: 495nm
Emission: 519nmPhysical formLiquidStorage and StabilityStore BioTracker 488 Green CSFE Cell Proliferation Kit -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. Ideally the 5 mM DMSO stock solution should be prepared on the day of use. Aliquots may be stored for later use, but activity may be reduced over time. The dyes should only be added to aqueous buffer immediately before staining.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient Узнать цену -
BioTracker 488 Green Lipid Droplet Dye SCT144
General descriptionLipids are fundamental building blocks of cells and play important cellular roles. Lipids are key components of the plasma membrane and other cellular compartments, including the nuclear membrane, endoplasmic reticulum, Golgi apparatus, and trafficking vesicles such as endosomes and lysosomes. Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. Lipids have been implicated in various diseases including heart, vascular and neurological diseases.
BioTracker Lipid dyes rapidly stain lipid droplets in live or fixed cells with no wash step and minimal background staining of cellular membranes or other organelles. Cells also can be fixed and permeabilized after staining.
The BioTracker 488 Green Lipid Dye has an excitation around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence using the FITC detection channel.
Spectral Properties
Absorbance: 427nm
Emission: 585nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging dye that rapidly stains lipid droplets with no wash steps and minimal background staining of other cellular membranes or organelles.QualitySpectral Properties
Absorbance: 427nm
Emission: 585nmPhysical formLiquid, supplied as a 1000X solution in DMSOStorage and StabilityStore BioTracker 488 Green Lipid Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F - (refers to pure substance) Flash Point C 87 °C - (refers to pure substance) Узнать цену -
BioTracker 488 Green Microtubule Cytoskeleton Dye SCT142
General descriptionCytoskeleton proteins such as actin and tubulin play an important role in cell structure and motility. Dysregulation of cytoskeletal proteins are also involved in cancer metastasis and disease. Taxol binds to polymerized tubulin and stabilizes microtubules, resulting in inhibition of mitosis. However, fluorescent taxol compounds like the BioTracker Microtubule Cytoskeleton dyes are less disruptive of microtubule dynamics and cell division, presumably due to lower binding affinity of the fluorescent probe compared to taxol itself.
The BioTracker Cytoskeleton Microtubule Dye is a green fluorescent cell-permeable taxol probe for imaging the microtubule cytoskeleton in live cells. It is a simple, rapid and sensitive stain that can be detected using the FITC channel. The dye is supplied with a vial of 100mM verapamil, an efflux pump inhibitor that may improve probe retention and staining in certain cell types.
Spectral Properties
Absorbance: 500nm
Emission: 515nmApplicationResearch Sub Category
Live Cell Dye
Live cell fluorescent imaging
Live cell imaging microtubule dye for imaging the cytoskeleton of living cells
Research Category
Cell SignalingComponents1) 1 vial of 50µL BioTracker 488 Green Microtubule Cytoskeleton Dye (1000X in DMSO) (CS224593)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)QualitySpectral Properties
Absorbance: 500nm
Emission: 515nmPhysical formLiquidStorage and StabilityStore BioTracker 488 Green Microtubule Cytoskeleton Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety Informationpictograms GHS07,GHS09 signalword Warning pcodes P273 Узнать цену -
BioTracker 488 Green Mitochondria Dye SCT136
General descriptionMitochondria are organelles found in large numbers in most cells, in which the biochemical processes of respiration and energy production occurs. In metabolically active cells, mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.
BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is lost when mitochondria become depolarized during cell death, allowing monitoring of cell viability.
The BioTracker 488 Green Mitochondria Dye can be detected using the green FITC/GFP channel. Also, the green staining is not dependent on membrane potential, and can be used to stain both live and fixed cells.
Spectral Properties
Absorbance: 490nm
Emission: 523nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health.QualitySpectral Properties
Absorbance: 490nm
Emission: 523nmPhysical formLyophilizedStorage and StabilityStore BioTracker 488 Green Mitochondria Dye at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
BioTracker 488 Green Nuclear Dye SCT120
General descriptionThe nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cells genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Note: BioTracker Nuclear Dyes also shows blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.
Spectral Properties
Absorbance: 500nm
Emission: 515nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.Components1) 1 vial of 50µL BioTracker 488 Green Nuclear Dye (1000X in DMSO) (CS224594)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)QualitySpectral Properties
Absorbance: 500nm
Emission: 515nmPhysical formLiquidStorage and StabilityStore BioTracker 488 Green Nuclear Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationPictograms GHS07,GHS09 Signal Word Warning Precautionary Statements P273 Узнать цену -
BioTracker 490 Green Cytoplasmic Membrane Dye SCT106
General descriptionLipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 484nm
Emission: 501nmApplicationResearch Sub Category
Live Cell Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Live cell fluorescent imagingQualitySpectral Properties
Absorbance: 484nm
Emission: 501nmPhysical formLiquidStorage and StabilityStore BioTracker 490 Green Cytoplasmic Membrane Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationPictograms GHS02,GHS07 Signal Word Danger Hazard Statements H225 - H319 Precautionary Statements P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 WGK Germany WGK 1 Узнать цену -
BioTracker 510 Green -GlcU(Na) Dye SCT027
eCl@ss: 32161000 General description-glucuronidase (-GlcU) is a type of glucuronidase that catalyzes hydrolysis of -D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. -Glucuronidase (GUSB) is an important lysosomal enzyme and aids in lysosomal storage and function.
The BioTracker 510 Green -GlcU(Na) is permeable through the cell membrane and is a fluorescent substrate for detecting -glucuronidase in living cells. Non-fluorescent dye is hydrolyzed by -glucuronidase, and generates bright green fluorescence (510 nm) when it is irradiated by the 490 nm excitation light.
Spectral Properties
Absorbance: 492nm
Emission: 510nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 510 Green -GlcU(Na) Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F - (Dimethylsulfoxide) Flash Point C 87 °C - (Dimethylsulfoxide) Узнать цену -
BioTracker 510 Green -Glu Dye SCT026
eCl@ss: 32161000 General descriptionBeta-glucosidase (-Glu) catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and oligosaccharides, with release of glucose.
The BioTracker 510 Green -Glu Dye is permeable through the cell membrane and is fluorescent substrate [9- (4-methoxy-2-methylphenyl) -6- (-D-glucopylanosyloxy) -xanthen-3-one] for detecting -glucosidase in living cells. Non-fluorescent dye is hydrolyzed by the -Glucosidase, and generates bright green fluorescence (510 nm) when it is irradiated by the 490 nm excitation light.
Spectral Properties
Absorbance: 492nm
Emission: 510nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 510 Green -Glu Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 176.0 °F - (Dimethylsulfoxide) Flash Point C 80 °C - (Dimethylsulfoxide) Узнать цену -
BioTracker 510 Green C2(FM2-10) Synaptic Dye SCT132
General descriptionBioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.
BioTracker 510 Green C2(FM2-10) Synaptic Dye is more water soluble than BioTracker 510 Green C4(FM1-43) Synaptic Dye and thus has a faster de-staining rate.
Spectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm
Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.QualitySpectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm
Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nmPhysical formLyophilizedStorage and StabilityStore BioTracker 510 Green C2(FM2-10) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 510 Green C4(FM1-43) Synaptic Dye SCT126
General descriptionBioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.
Spectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm
Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.QualitySpectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm
Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nmPhysical formLyophilizedStorage and StabilityStore BioTracker 510 Green C4(FM1-43) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 515 Green ONOO- Dye SCT035
eCl@ss: 32161000 General descriptionPeroxynitrite (ONOO-) is a strong oxidant generated from the interaction of nitric oxide (NO) and superoxide (O). The in vivo formation of this compound has recently been demonstrated in macrophages, neutrophils and endothelial cells. The role of ONOO- in inflammatory diseases is further supported by the detection of immunoreactive nitrotyrosine on proteins at sites of inflammation.
The BioTracker 515 Green ONOO- Dye is a fluorescent probe that detects peroxynitrite (ONOO-) in living cells. Fluorescent intensity is not increased by the existence of hydroxyl radical, singlet oxygen, hydrogen peroxide, hypochlorite, nitric oxide or superoxide.
Spectral Properties
Absorbance: 490nm
Emission: 515nmStorage and StabilityStore BioTracker 515 Green ONOO- Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 515 Green Zn2+ DA Dye SCT032
eCl@ss: 32161000 General descriptionZinc (Zn2+) is a trace nutrient critical for physiological function. Although most biological zinc ions are tightly associated with proteins, pools of loosely bound or mobile forms serve regulatory or signaling functions, including nucleation of protein self-assembly, triggering of signaling pathways, and modification of cellular metabolism.
The BioTracker 515 Green Zn2+ Dye is a fluorescent imaging probe for the detection of Zn2+ in living cells. The BioTracker 515 Green Zn2+ Dye has high solubility, so that it is not permeable through the cell membrane. BioTracker 515 Green Zn2+ Dye DA has an additional acetyl group which make the reagent go inside the cell much easier, and the acetyl group is removed by the intracellular esterase which make the reagent stay inside the cell for extended periods of time.
Concentration of the provided sample is 5 mmol/L in DMSO. Dilute 1000 times with neutral buffer before use.
Spectral Properties
Absorbance: 492nm
Emission: 515nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 515 Green Zn2+ DA Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 849.6 °F Flash Point C 454.2 °C Узнать цену -
BioTracker 515 Green Zn2+ Dye SCT031
eCl@ss: 32161000 General descriptionZinc (Zn2+), is a trace nutrient critical for physiological function. Although most biological zinc ions are tightly associated with proteins, pools of loosely bound or mobile forms serve regulatory or signaling functions, including nucleation of protein self-assembly, triggering of signaling pathways, and modification of cellular metabolism.
The BioTracker 515 Green Zn2+ Dye is a fluorescent imaging probe for the detection of Zn2+ in living cells. The BioTracker 515 Green Zn2+ Dye has high solubility, so that it is not permeable through the cell membrane. BioTracker 515 Green Zn2+ Dye DA has an additional acetyl group which make the reagent go inside the cell much easier, and the acetyl group is removed by the intracellular esterase which make the reagent stay inside the cell for extended periods of time.
Concentration of the provided sample is 5 mmol/L in DMSO. Dilute 1000 times with neutral buffer before use.
Spectral Properties
Absorbance: 492nm
Emission: 515nmStorage and StabilityStore BioTracker 515 Green Zn2+ Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 871.7 °F Flash Point C 466.5 °C Узнать цену -
BioTracker 519 Green -Gal Dye SCT025
eCl@ss: 32161000 General description-galactosidase, also called beta-gal or -gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of -galactosides into monosaccharides through the breaking of a glycosidic bond. -gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. -gal has also been used to measure cellular senescence (SA--gal).
The BioTracker 543 Green -Gal Dye is a fluorescent probe for the detection of -galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. The dye can stain LacZ expressing HEK293 cells both before and after 3% paraformaldehyde fixation.
Spectral Properties
Absorbance: 497nm
Emission: 519nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 543 Green -Gal Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before openingDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 520 Green Hypoxia Dye SCT033
eCl@ss: 32161000 General descriptionHypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells. Hypoxia in living tissue closely relates to a wide array of pathologies, including cancer and ischemia. Existing hypoxia detection probes, such as pimonidazole, require cell fixation followed by immunostaining.
The BioTracker 520 Green Hypoxia Dye is a fluorescent imaging probe for the detection of hypoxia in living cells. This probe is as sensitive as pimonidazole and can be used for live cell fluorescence imaging and flow cytometry applications.
Spectral Properties
Absorbance: 498nm
Emission: 520nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 520 Green Hypoxia Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
BioTracker 525 Green GGT Dye SCT028
eCl@ss: 32161000 General descriptionGamma-glutamyltransferase, or GGT, is an enzyme that plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione and drug and xenobiotic detoxification. GGT is predominantly used as a diagnostic marker for liver disease and is often significantly increased in human malignancies and cancers.
The BioTracker 525 Green GGT Dye is cancer-selective fluorescent imaging probe. This probe is rapidly activated by g-glutamyltranspeptidase (GGT), which is known to be tumor-associated enzyme, to give highly fluorescent compound. The membrane-permeable dye is applicable to live cell or in vivo imaging of cancer cells.
Spectral Properties
Absorbance: 496nm
Emission: 525nmStorage and StabilityStore BioTracker 525 Green GGT Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 529 Green Pluripotent Stem Cell Dye SCT029
eCl@ss: 32161000 General descriptionPluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). Characterization of human iPS cells includes alkaline phosphatase and antibody staining for Oct-4, Sox-2, Nanog, SSEA and TRA surface markers. These assays require cell fixation and are end-point assays.
The BioTracker 529 Green Pluripotent Stem Cell Dye is a live stem cell probe that selectively identifies undifferentiated human ES and iPS cells. The probe localizes at mitochondria in the human stem cells. The dye’s fluorescence is dependent on the activities of ABCB1 and ABCG2 on the cell membrane. The dye is suitable for both live cell imaging and flow cytometry applications of human pluripotent stem cells.
Spectral Properties
Absorbance: 515nm
Emission: 529nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 529 Green Pluripotent Stem Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 543 Yellow -Gal Dye SCT024
eCl@ss: 32161000 General description-galactosidase, also called beta-gal or -gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of -galactosides into monosaccharides through the breaking of a glycosidic bond. -gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. -gal has also been used to measure cellular senescence (SA--gal).
The BioTracker 543 Yellow -Gal Dye is a fluorescent probe for the detection of -galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of -galactosidase, this probe exhibits good S/N ratio.
Spectral Properties
Absorbance: 525nm (488nm compatible)
Emission: 543nmStorage and StabilityStore BioTracker 543 Yellow -Gal Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 555 Orange Cytoplasmic Membrane Dye SCT107
General descriptionLipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 549nm
Emission: 565nmApplicationResearch Sub Category
Live Cell Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Live cell fluorescent imagingQualitySpectral Properties
Absorbance: 549nm
Emission: 565nmPhysical formLiquidStorage and StabilityStore BioTracker 555 Orange Cytoplasmic Membrane Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety Informationpictograms GHS02,GHS07 signalword Danger hcodes H225 - H319 pcodes P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 WGK Germany WGK 1 Flash Point F 55.4 °F Flash Point C 13 °C Узнать цену -
BioTracker 555 UV-Excitation Red Lysosome Dye SCT140
General descriptionLysosomes are membrane-enclosed organelles that contain an array of enzymes capable of breaking down all types of biological material including proteins, nucleic acids, carbohydrates, and lipids. Lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself.
BioTracker Lysosome dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step.
The BioTracker 555 UV-Excitation Red Lysosome is a red fluorogenic lysosome dye with pH-dependent fluorescence. The dye is unique among commercially available lysosome dyes in that its fluorescence in cells is activated by exposure to UV excitation. In solution, the dye shows pH-dependent fluorescence that does not require UV activation. The dye initially shows low fluorescence, but brief exposure to UV excitation from a mercury arc lamp through a DAPI filter.
Spectral Properties
Absorbance: 554nm
Emission: 583nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging dye for acidic cellular organelles such as lysosomes.QualitySpectral Properties
Absorbance: 554nm
Emission: 583nmPhysical formLiquidStorage and StabilityStore BioTracker 555 UV-Excitation Red Lysosome Dye at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F - (refers to pure substance) Flash Point C 87 °C - (refers to pure substance) Узнать цену -
BioTracker 560 Orange Lysosome Dye SCT019
eCl@ss: 32161000 General descriptionLysosomes are membrane-enclosed organelles that contain an array of enzymes capable of breaking down all types of biological material including proteins, nucleic acids, carbohydrates, and lipids. Lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself.
The BioTracker 560 Orange Lysosome Dye is a live cell fluorescence imaging probe which enhances fluorescence dramatically in acidic environments. This probe can stain acidic organelles such as lysosome, late endosome and granule selectively. Namely, in the condition of pH 5.0, identical to the environment of acidic organelles, fluorescence intensity is 50-fold or more compared to physical pH 7.4. The BioTracker 560 Orange Lysosome Dye shows great photostability against irradiating excitation light. As it emits orange color fluorescent by excitation at 532nm or 514 nm, multicolor imaging is possible by combining with blue fluorescence (CFP, Hoechst, etc.), green fluorescence (GFP, fluorescein, etc.) and near-infrared fluorescence.
Spectral Properties
Absorbance: 535nm
Emission: 560nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 560 Orange Lysosome Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 574 Red HOCL Dye SCT034
eCl@ss: 32161000 General descriptionThe production of hypochlorous acid (HOCl) by the myeloperoxidase-H2O2-Cl- system of phagocytes plays a vital role in the ability of immune cells to kill a wide range of pathogens including bacteria and viruses. Hypochlorous acid is one of the various reactive oxygen species (ROS) including hydroxyradical (OH), superoxide (O2-), hydrogen peroxide (H2O2), singlet oxygen (1O2), nitric oxide (NO), and peroxynitrite (ONOO-).
The BioTracker 574 Red HOCL Dye is a fluorescent probe that shows excellent selectivity against hypochlorous acid (HOCl) in living cells. The dye contains fast reactivity, slow photobleaching and is suitable for time-lapse live cell imaging.
Spectral Properties
Absorbance: 553nm
Emission: 574nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 574 Red HOCL Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety Informationhcodes H413 pcodes P273 - P501 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 575 Red Fe2+ Dye SCT030
eCl@ss: 32161000 General descriptionIron (Fe2+) is an essential element for blood production. About 70 percent of your bodys iron is found in the red blood cells of your blood called hemoglobin and in muscle cells called myoglobin. Iron is an essential metal for living organisms, but dysregulation of its homeostasis at the cellular level can trigger detrimental oxidative and/or nitrosative stress and damage events.
The BioTracker 575 Red Fe2+ Dye is a fluorescent imaging probe for the detection of iron (II) in living cells. This probe is localized in Golgi, and reacts selectively with Fe2+.
Spectral Properties
Absorbance: 540nm
Emission: 575nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 575 Red Fe2+ Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 609 Red Ca2+ AM Dye SCT021
eCl@ss: 32161000 General descriptionCalcium (Ca2+) play a vital role in the physiology and biochemistry of the cell. Calcium ions play an important role in signal transduction pathways, neurotransmitter release from neurons, contraction of all muscle cell types, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.
The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.
Spectral Properties
Absorbance: 595nm
Emission: 609nmStorage and StabilityStore BioTracker 609 Red Ca2+ AM Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F 1202.4 °F Flash Point C 650.2 °C Узнать цену -
BioTracker 625 Red GSH Dye SCT036
eCl@ss: 32161000 General descriptionGlutathione (GSH) is an important antioxidant preventing damage to important cellular components caused by reactive oxygen species (ROS) including free radicals, peroxides, lipid peroxides, and heavy metals. Alterations in glutathione homeostasis are associated with a variety of diseases; therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes.
The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells. The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S). At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light. At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T. The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity.
Spectral Properties
Absorbance: 520nm
Emission: 625nm and 582nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingPhysical formLyophilizedStorage and StabilityStore BioTracker 625 Red GSH Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 633 Red Mitochondria Dye SCT137
General descriptionMitochondria are organelles found in large numbers in most cells, in which the biochemical processes of respiration and energy production occurs. In metabolically active cells, mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.
BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is lost when mitochondria become depolarized during cell death, allowing monitoring of cell viability.
The BioTracker 633 Red Mitochondria Dye can be detected using the red Cy5 channel. The fluorescence is dependent on mitochondrial membrane potential, and the dye can be used to monitor changes in mitochondrial membrane potential in cells during apoptosis.
Spectral Properties
Absorbance: 622nm
Emission: 648nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health.QualitySpectral Properties
Absorbance: 622nm
Emission: 648nmPhysical formLyophilizedDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker 640 Red C2(FM4-64) Synaptic Dye SCT127
General descriptionBioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.Quality
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nmPhysical formLyophilizedStorage and StabilityStore BioTracker 640 Red C2(FM4-64) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
BioTracker 650 Red Nuclear Dye SCT119
General descriptionThe nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cells genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker 650 Red Nuclear Dye is a cell-membrane permeable red fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Spectral Properties
Absorbance: 650nm
Emission: 675nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.Components1) 1 vial of 50µL BioTracker 650 Red Nuclear Dye (1000X in DMSO) (CS224577)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)QualitySpectral Properties
Absorbance: 650nm
Emission: 675nmPhysical formLiquidStorage and StabilityStore BioTracker 650 Red Nuclear Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationPictograms GHS07,GHS09 Signal Word Warning Precautionary Statements P273 Узнать цену -
BioTracker 655 Red Cytoplasmic Membrane Dye SCT108
General descriptionLipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 644nm
Emission: 665nmApplicationResearch Sub Category
Live Cell Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Live cell fluorescent imagingQualitySpectral Properties
Absorbance: 644nm
Emission: 665nmPhysical formLiquidStorage and StabilityStore BioTracker 655 Red Cytoplasmic Membrane Dye at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationPictograms GHS02,GHS07 Signal Word Danger Hazard Statements H225 - H319 Precautionary Statements P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 WGK Germany WGK 1 Узнать цену -
BioTracker ATP-Red Live Cell Dye SCT045
General descriptionAdenosine triphosphate (ATP) is the primary energy source for all cellular processes. ATP also functions as a signaling molecule for regulating cell movement, neurotransmission and ion channel functions. ATP is localized in mitochondria, where cellular respiration occurs. ATP levels can be used to measure cell proliferation and cell cycle dynamics.
The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence.
Spectral Properties
Absorbance: 510nm
Emission: 570nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingQualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 561.48 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker ATP-Red Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
BioTracker CyP AP Live Cell Dye SCT046
General descriptionAlkaline Phosphatase (AP) catalyzes the hydrolysis of the phosphate group in alkaline media. AP is an essential enzyme and widely distributes in a variety of tissues such as liver, intestine, kidney, bone, and placenta. Abnormal AP levels have been shown in numerous cancers, bone disease and liver dysfunction. AP is also expressed by pluripotent stem cells such as ES and IPS cells.
The BioTracker CyP AP dye is a live cell near-infrared (NIR) fluorescent imaging probe for alkaline phosphatase. With its long emission wavelength, the probe is suitable for monitoring AP levels in both in vivo and in vitro tissues and cells. The probe possesses deep tissue penetration, less damage to the biological samples, and lower interference from the background fluorescence compared to other AP probes.
Spectral Properties
Absorbance: 690nm
Emission: 738nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingQualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 528.55 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker CyP AP Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker Cystine-FITC Live Cell Dye SCT047
General descriptionUptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.
The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.
Spectral Properties
Absorbance: 450nm
Emission: 525nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingQualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 625.65 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker LYSO-TP Live Cell Dye SCT044
General descriptionHypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.
The BioTracker LYSO-TP dye is a live cell two-photon green fluorescent turn-on imaging probe for HOCL. The probe targets HOCL specifically in the lysosomes of cells. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical pushpull (amineketone) structure. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement. To monitor subcellular HOCL levels, morpholine was introduced at a long distance from the reaction site to ensure the probes intracellular localization.
Spectral Properties
Absorbance: 375nm
Emission: 500nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imagingQualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 357.49 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker LYSO-TP Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker NIR633 Lysosome Dye SCT138
General descriptionLysosomes are membrane-enclosed organelles that contain an array of enzymes capable of breaking down all types of biological material including proteins, nucleic acids, carbohydrates, and lipids. Lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself.
BioTracker Lysosome dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step.
The BioTracker NIR633 Lysosome Dye can be detected using the red Cy5 channel. The fluorescence is dependent on lysosome pH changes.
Spectral Properties
Absorbance: 634nm
Emission: 659nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging dye for acidic cellular organelles such as lysosomes.QualitySpectral Properties
Absorbance: 634nm
Emission: 659nmPhysical formLiquidStorage and StabilityStore BioTracker NIR633 Lysosome Dye at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker NIR694 Nuclear Dye (DMSO) SCT118
General descriptionThe nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cells genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker NIR694 Nuclear Dye (DMSO) is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. Dye staining is nuclear specific in fixed and permeabilized cells and tissue sections and does not require RNase treatment. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. For live cell nuclear staining we recommend the BioTracker NIR694 Nuclear Dye (Water).
Spectral Properties
Absorbance: 662nm
Emission: 694nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. The BioTracker NIR694 Nuclear Dye (DMSO) is supplied as a 200X stock solution in DMSO.QualitySpectral Properties
Absorbance: 662nm
Emission: 694nmPhysical formLiquidStorage and StabilityStore BioTracker NIR694 Nuclear Dye (DMSO) at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 2 Flash Point F 188.6 °F - (refers to pure substance) Flash Point C 87 °C - (refers to pure substance) Узнать цену -
BioTracker NIR694 Nuclear Dye (Water) SCT117
General descriptionThe nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cells genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.QualitySpectral Properties
Absorbance: 662nm
Emission: 694nmPhysical formLiquidStorage and StabilityStore BioTracker NIR694 Nuclear Dye (Water) at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 2 Flash Point F does not flash Flash Point C does not flash Узнать цену -
BioTracker NTP-Transporter Molecule SCT064
General descriptionNucleoside triphosphates (NTPs) are important metabolites that are involved in numerous cellular processes. However, due to their anionic nature, NTPs do not readily permeate cell membranes. To overcome this barrier, synthetic analogues that are neutral and more readily transportable are commonly used in cell-based assays. However, these synthetic analogues have a very low efficiency of conversion to active metabolites. Hence there is a need to optimize transport of NTPs into the cells.
BioTracker<TMSYMBOL></TMSYMBOL> NTP-Transporter Molecule has a receptor site, for selectively binding to the NTPs, and a cell penetrating agent. The transporter can bind to NTPs and quickly move them into cells without any apparent damage to the cell membrane or decrease in cell viability. The transported NTP is rapidly liberated from the complex and available in the nuclei for incorporation into DNA. Labeled NTPs can be used for live cell imaging, cell proliferation assays, synthetic biology studies, synthesizing artificial DNA, tracking cell division, research on active antiviral and anticancer nucleotides, among other things.ApplicationResearch Sub Category
Cell Cycle, DNA Replication & Repair
DNA Damage & Repair
Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.
Research Category
Cell Imaging
Live cell fluorescent imagingQualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 3470.14 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker NTP-Transporter Molecule at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Other NotesConcentration: Please refer to lot specific datasheet.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker NucView® 405 Blue Caspase-3 Dye (DMSO) SCT102
General descriptionCaspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 405 Blue Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 405 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright blue fluorescence. Thus, NucView 405 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
NucView 405 dye is excited by the 405nm laser line for detection by confocal microscopy in the DAPI channel, or by flow cytometry in the Pacific Blue channel. NucView 405 is ideal for caspase-3 detection in multi-color applications for researchers who wish to reserve the green fluorescence channel for other detection reagents.
Spectral Properties
Absorbance: 429nm
Emission: 469nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.QualitySpectral Properties
Absorbance: 429nm
Emission: 469nmPhysical formLiquidStorage and StabilityStore BioTracker NucView 405 Blue Caspase-3 Dye, 1mM in DMSO at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Legal InformationNucView is a registered trademark of Biotium Inc.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F - (refers to pure substance) Flash Point C 87 °C - (refers to pure substance) Узнать цену -
BioTracker NucView® 405 Blue Caspase-3 Dye (PBS) SCT104
General descriptionCaspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 405 Blue Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 405 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright blue fluorescence. Thus, NucView 405 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
NucView 405 dye is excited by the 405nm laser line for detection by confocal microscopy in the DAPI channel, or by flow cytometry in the Pacific Blue channel. NucView 405 is ideal for caspase-3 detection in multi-color applications for researchers who wish to reserve the green fluorescence channel for other detection reagents.
Spectral Properties
Absorbance: 429nm
Emission: 469nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.QualitySpectral Properties
Absorbance: 429nm
Emission: 469nmPhysical formLiquidStorage and StabilityStore BioTracker NucView 405 Blue Caspase-3 Dye, 1 mM in PBS at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Legal InformationNucView is a registered trademark of Biotium Inc.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F does not flash Flash Point C does not flash Узнать цену -
BioTracker NucView® 488 Green Caspase-3 Dye (DMSO) SCT100
General descriptionCaspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 488 Green Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 488 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView 488 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
Spectral Properties
Absorbance: 500nm
Emission: 530nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.QualitySpectral Properties
Absorbance: 500nm
Emission: 530nmPhysical formLiquidStorage and StabilityStore BioTracker NucView 488 Green Caspase-3 Dye, 1 mM in DMSO at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Legal InformationNucView is a registered trademark of Biotium Inc.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F - (refers to pure substance) Flash Point C 87 °C - (refers to pure substance) Узнать цену -
BioTracker NucView® 488 Green Caspase-3 Dye (PBS) SCT101
General descriptionCaspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 488 Green Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 488 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView 488 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
Spectral Properties
Absorbance: 500nm
Emission: 530nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.QualitySpectral Properties
Absorbance: 500nm
Emission: 530nmPhysical formLiquidStorage and StabilityStore BioTracker NucView 488 Green Caspase-3 Dye, 1 mM in PBS at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Legal InformationNucView is a registered trademark of Biotium Inc.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F does not flash Flash Point C does not flash Узнать цену -
BioTracker NucView® 530 Red Caspase-3 Dye (DMSO) SCT103
General descriptionCaspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 530 Red Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 530 Red Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright red fluorescence. Thus, NucView 530 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
NucView 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence, for detection in the Cy3 channel by fluorescence microscopy or the PE channel by flow cytometry. Note that when excited by the 488nm laser line, NucView 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.
Spectral Properties
Absorbance: 528nm
Emission: 563nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.QualitySpectral Properties
Absorbance: 528nm
Emission: 563nmPhysical formLiquidStorage and StabilityStore BioTracker NucView 530 Red Caspase-3 Dye, 1mM in DMSO at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Legal InformationNucView is a registered trademark of Biotium Inc.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F - (refers to pure substance) Flash Point C 87 °C - (refers to pure substance) Узнать цену -
BioTracker NucView® 530 Red Caspase-3 Dye (PBS) SCT105
General descriptionCaspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.
The BioTracker NucView 530 Red Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 530 Red Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright red fluorescence. Thus, NucView 530 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.
NucView 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence, for detection in the Cy3 channel by fluorescence microscopy or the PE channel by flow cytometry. Note that when excited by the 488nm laser line, NucView 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.ApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.QualityAbsorbance Spectrum: 523 +/- 5
TLC: >95%Physical formLiquidStorage and StabilityStore BioTracker NucView 530 Red Caspase-3 Dye, 1mM in PBS at -20C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Legal InformationNucView is a registered trademark of Biotium Inc.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыapplication(s) cell analysis: suitable (cell based assays) detection method fluorometric shipped in ambient
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker QUMA-1 RNA G-quadruplex Live Cell Dye SCT056
General descriptionRNA G-quadruplexes are formed by self-assembly of guanine-rich RNA stacks into stacked G-quartets. This can occur in protein-coding (mRNA) as well as non-coding (ncRNA) transcripts. There is growing evidence that RNA G-quadruplexes are involved in different biological functions: telomere elongation, DNA recombination, transcription, RNA post-transcriptional mechanisms (pre-mRNA processing, mRNA turnover, targeting and translation), and are associated with sevral human diseases.
BioTracker<TMSYMBOL></TMSYMBOL> QUMA-1 RNA G-quadruplex Live Cell Dye (QUMA-1) is a red-emitting fluorescent probe that can be used to study dynamics (folding and unfolding) and movements of RNA G-quadruplexes. QUMA-1 is a reversible dye that emits upon binding to G-quadruplexes and turns off upon unfolding. QUMA-1 is highly specific for RNA G-quadruplexes and does not bind to DNA, double-stranded RNA, single-stranded RNA or other RNA structures. QUMA-1 could also potentially be used to study RNA-protein interactions.
Spectral Properties
Absorbance: 555 nm
Emission: 660 nmApplicationResearch Sub Category
Live Cell Dye
Live cell fluorescent imaging
A red-emitting fluorescent probe for tracking RNA G-quadruplexes dynamics, such as folding and unfolding, in live cells.
Research Category
Cell Imaging
microRNA
RNA Metabolism & Binding ProteinsQualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 628.17 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker QUMA-1 RNA G-quadruplex -Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Other NotesConcentration: Please refer to lot specific datasheet.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BioTracker Si-DMA Singlet Oxygen Live Cell Dye SCT063
General descriptionSinglet oxygen (1O2) is one of the reactive oxygen species (ROS). Singlet oxygen is known to be a cause of spots and wrinkles of the skin due to its very strong oxidizing potential. However, the reactivity of singlet oxygen, if controlled, can be used for therapy. In the field of cancer research and therapy, singlet oxygen is being used for photodynamic therapy (PDT), an emerging anticancer treatment using photoirradiation and photosensitizers.
BioTracker<TMSYMBOL></TMSYMBOL> Si-DMA Singlet Oxygen Live Cell Dye is a novel far-red fluorescence probe that is composed of silicon-containing rhodamine and anthracene moieties as a chromophore and a singlet oxygen reactive site, respectively. In the presence of singlet oxygen, fluorescence of BioTracker Si-DMA live cell dye increases 17 times due to endoperoxide formation at the anthracene moiety. The dye is highly selective to singlet oxygen, among seven different reactive oxygen species (1O2, O2-, H2O2, HOCL-, RCO-, OH-, NO). Among three different intracellular photosensitizers, BioTracker Si-DMA selectively detects the 1O2 that is generated by 5-aminolevulinic acid-derived protoporphyrin IX, localized in mitochondria. On the other hand, mitochondria-targeted KillerRed and lysosomal porphyrins could not induce fluorescence change in the dye.
Spectral Properties
Absorbance: 640 nm
Emission: 670 nmApplicationResearch Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging
Live cell imaging dye for singlet oxygen (1O2) localized to mitochondria. Singlet oxygen is important in the field of cancer therapy, photodynamic medicine and other skin treatments.QualityPurity: 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 513.77 g/molPhysical formLyophilizedStorage and StabilityStore BioTracker Si-DMA Singlet Oxygen Live Cell Dye at 0-5°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.Other NotesConcentration: Please refer to lot specific datasheet.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыassay 98% (H-NMR), 98% (HPLC), 98% (LC/MS), 98% (elemental analysis) application(s) cell analysis: suitable (cell based assays) detection method fluorometric
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
